NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed.
Storage Temp
Protected from light,Store at -20°C,Desiccated
Shipped In
Ice chest + Ice pads
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Product Description
NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed.
In Vitro
NSP-SA-NHS marking?\n(1) 1mg BSA was dissolved in 0.3 mL PBS (solution A), and 0.2 mg NSP-SA-NHS was dissolved in 40 μL DMF (solution B). ?\n(2) Add solution B to solution A, mix at 18°C, 130 rpm, and dark for 12 h. ?\n(3) The mixture was dialyzed with 500 mL of distilled water at 4°C for 2 d, during which distilled water was changed twice (solution C). ?\n(4) Slowly add solution B to solution C, mix at 18°C, 130 rpm, and dark for 24 h, and then dialysis with distilled water for 48 h. During this period, change distilled water four times. ?\n(5) Dilute the coupling compound with distilled water to a suitable concentration, and analyze it with an ultraviolet spectrophotometer. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
This compound belongs to the class of organic compounds known as acridines. These are organic compounds containing the acridine moiety, a linear tricyclic heterocycle which consists of two benzene rings joined by a pyridine ring.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
K2404440