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NQO1 Mouse mAb - Primary antibody, specific to Human NQO1

Grade & Purity:

≥95%(SDS-PAGE)
1.0 mg/mL

Application:

ELISA
Flow Cytometry
IF/ICC
WB

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG2b
Clone Number: AB02/1F11
Conjugation: Unconjugated

In stock

Item Number

Ab224768

Grouped product items
SKU	Size	Availability	Price
Ab224768-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab224768-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$199.90
Ab224768-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$319.90
Ab224768-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

mAb (AB02/1F11); Mouse anti Human NQO1; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated

View related series

Click to view target information: NQO1

Accession#:P15559 (5) Gene ID: 1728 (2) NQO1 (5)

Basic Description

Product Name	NQO1 Mouse mAb - Primary antibody, specific to Human NQO1
Specifications & Purity	Carrier Free, Endotoxin Free, Validated, High Performance, ExactAb™, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms	Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofac
Host species	Mouse
Specificity	Human NQO1
Immunogen	Recombinant full length protein corresponding human NQO1
Positive Control	WB: HepG2, MCF-7, HeLa and A549 whole cell lysates Flow Cytometry: MCF-7 cells IF/ICC: MCF-7 cells
Conjugation	Unconjugated
Grade	Carrier Free, Endotoxin Free, ExactAb™, High Performance, Validated
Product Description	Mouse anti Human NQO1 Antibody, clone AB01/1F11, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type	kappa
SDS-PAGE	150.0 KDa
Purification Method	Protein A purified
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

NQO1 Tchem NAD(P)H dehydrogenase [quinone] 1 (0 Activities)

Discover Target: NQO1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: NQO1 Mouse mAb (Ab224768)

NQO1 Mouse mAb (Ab224768) - Western Blot
All lanes: NQO1 Mouse Antibody (Ab224768) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 30 kDa
Exposure time: 30.0 s

Flow Cytometry: NQO1 Mouse mAb (Ab224768)

NQO1 Mouse mAb (Ab224768) - Flow Cytometry 
Overlay histogram showing MCF-7 cells stained with NQO1 Mouse mAb (Ab224768) (Red) at 1.0 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).

IF: NQO1 Mouse mAb (Ab224768)

NQO1 Mouse mAb (Ab224768) - IF
IF analysis of NQO1 (Green) in MCF-7 cells. The cells were fixed with 4% PFA for 15 minutes and then 0.3% Triton X-100 for 15 minutes at RT. Cells were stained with NQO1 Mouse mAb (Ab224768) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IF/ICC	1/200
WB	1/1000 - 1/200000
Flow Cytometry	1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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