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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab215220-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab215220-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab215220-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
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Ab215220-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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mAb (AB01/1C6); Mouse anti Human NQO1; WB, Flow Cytometry, ELISA; Unconjugated
| Product Name | NQO1 Mouse mAb - Primary antibody, specific to Human NQO1 |
|---|---|
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofac |
| Host species | Mouse |
| Specificity | Human NQO1 |
| Immunogen | Recombinant full length protein corresponding human NQO1 |
| Positive Control | WB: A549, HeLa, MCF-7 and HepG2 whole cell lysates Flow Cytometry: MCF-7 cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human NQO1 Antibody, clone AB01/1C6, could be used for WB, Flow Cytometry, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
NQO1 Mouse mAb (Ab215220) - Western Blot
All lanes: NQO1 Mouse Antibody (Ab215220) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 30 kDa
Exposure time: 30.0 s
NQO1 Mouse mAb (Ab215220) - Flow Cytometry
Overlay histogram showing MCF-7 cells stained with NQO1 Mouse mAb (Ab215220) (Red) at 1.0 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).
| Application | Dilution info |
|---|---|
| WB | 1/500-1/100000 |
| Flow Cytometry | 1/500 |