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Nitric oxide assay kit

COA

In stock

Item Number

N598382

Grouped product items
SKU	Size	Availability	Price	Qty
N598382-200T	200T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$139.90
N598382-1000T	1000T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$489.90

View related series

Kit (35) No detection (1) Signal molecule detection (1)

Basic Description

Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	Product introduction： Griess reagent can be used for spectrophotometric detection of nitrite. The reagent contains two chemicals, sulfonic acid and n- (1-naphthyl) ethylenediamine. Under acidic conditions, sulfamic acid is converted into diazonium salt by nitrite, which can form a highly colored azo dye with n- (1-naphthyl) ethylenediamine. This dye can be detected at 548 nm: because no is extremely unstable, it is oxidized to form nitrite and nitrate. Griess indirectly reflects the content of no by detecting the content of nitrite. Matters needing attention： 1. before using Griess reagent, return it to room temperature and check the solution for precipitation. If Griess reagent I contains sediment when taken out, it can be placed in a 37 ℃ water bath until the sediment dissolves. 2. this product is potentially harmful. Avoid prolonged or repeated exposure. Avoid entering eyes, skin or clothing. Please wear lab clothes and disposable gloves for operation. Scope of application： No detection Component： Instruction： 1.Griess Reagent I and II were taken out to restore the room temperature. 2.Standard dilution : The standard NaNO2 ( 1-100 μM ) was diluted with the solution used for the sample to be tested. The standard was diluted to 1 μM, 10 μM, 20 μM, 40 μM, 80 μM and 100 μM, and 100 μL standard was added to each well. If the sample concentration is too low, the range of the standard curve can be appropriately reduced ( 1 μM, 2 μM, 3 μM, 4 μM, 6 μM, 8 μM, 10 μM ). 3.Sample detection : ( 1 ) According to the total volume of 200 μL / hole, 100 μL / hole sample was added to the 96-well plate ; if the sample is the supernatant of the culture medium, it can be sampled directly, and if there is sediment, the supernatant should be taken after centrifugation. If the sample is a cell or tissue, it can be quickly lysed by freeze-thaw, and then centrifuged to obtain the supernatant. The volume of less than 100 μL can be diluted with diH2O or 0.9 % NaCl ( corresponding standards also need to be diluted with diH2O or 0.9 % NaCl ). ( 2 ) According to 50 μL / hole, Griess Reagent I was added to each hole. ( 3 ) According to 50 μL / hole, Griess Reagent II was added to each hole. ( 4 ) The absorbance was measured at 540 nm. If there is no 540 nm filter, 520-560 nm filter can also be. If there is no microplate reader or a suitable filter, the concentration of nitric oxide in the sample can also be determined by visual colorimetry. A more precise concentration gradient is required for the standard when visual colorimetric.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
E2514351	Certificate of Analysis	Feb 25, 2025	N598382
C2406530	Certificate of Analysis	Dec 16, 2023	N598382

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