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NeutrAvidin Protein (Oregon Green 488)

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Bioactivity: Binding Biotin

In stock

Item Number

rp217318

Grouped product items
SKU	Size	Availability	Price
rp217318-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$11.90
rp217318-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
rp217318-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$214.90

Native; NeutrAvidin Protein (Oregon Green 488); Flow Cytometry; IF/ICC

View related series

NA (4) Neutral avidin (1) NeutrAvidin Protein (4) Oregon Green 488 (3)

Basic Description

Product Name	NeutrAvidin Protein (Oregon Green 488)
Synonyms	Nuetral Avidin \| NeutrAvidin
Grade	ExactAb™, Validated
Product Description	NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding. Features include: • Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin • Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin • No RYD recognition sequence—no known off-target binding domains like streptavidin • Affordable—significantly less expensive than streptavidin NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Bioactivity	Binding Biotin
Conjugation	废弃Lyophilized
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	526nm
Ex/Em(nm)
Predicted molecular weight	60kDa

Storage and Shipping

Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Images

IF: NeutrAvidin Protein (Oregon Green 488) (rp217318)

NeutrAvidin Protein (Oregon Green 488) (rp217318) - IF
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with NeutrAvidin Protein (Oregon Green 488) (rp217318) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: NeutrAvidin Protein (Oregon Green 488) (rp217318)

NeutrAvidin Protein (Oregon Green 488) (rp217318) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with NeutrAvidin Protein (Oregon Green 488) (rp217318) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	0.5-10μg/mL
IF/ICC	1-10μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

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