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Basic Description
| Product Name | Mouse brain tissue lysate - total protein |
|---|---|
| Product Description |
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE and WB analysis. The concentration was determined using the BCA assay process. |
| Specifications & Purity | 200μg |
| Species | BALB/c mice |
Storage and Shipping
| Reconstitution | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. |
|---|---|
| Storage Temp | Store at -80°C |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Shipped on Dry Ice. Lyophilizate is recommended stored at -80°C. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20°C for short-term storage or -80°C for long-term storage. |
Images
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: CAMK2B Mouse mAb (Ab092658) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 59 kDa
Exposure time: 49.2 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: ZnT-3 Antibody (Ab135449) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 6.6 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant PPP1CA/B Antibody (Ab122819) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 38, 37 kDa
Observed band size: 35 kDa
Exposure time: 36.5 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Quinone oxidoreductase Antibody (Ab124077) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 17.7 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant Haptoglobin Antibody (Ab008379) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 39 kDa
Exposure time: 23.5 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: MSC Antibody (Ab116083) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 28 kDa
Exposure time: 1.8 s
Mouse brain tissue lysate - total protein (tl155918) - Western Blot
All lanes: Recombinant Otx1 Antibody (Ab119495) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 100.0 s
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