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MHC class I/H-2Kb Mouse mAb (AF488) - Primary antibody, specific to MHC class I/H-2Kb

COA

Grade & Purity:

Validated

ExactAb™

0.5 mg/mL

Application:

Flow Cytometry

Host species: Mouse
Reactive species: Mouse
Isotype: Mouse IgG2a
Clone Number: AF6-88.5.5.3
Conjugation: AF488

In stock

Item Number

Ab190296

Grouped product items
SKU	Size	Availability	Price
Ab190296-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$39.90
Ab190296-25μg	25μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab190296-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$169.90

mAb (AF6-88.5.5.3); Mouse anti Mouse MHC class I/H-2Kb Antibody (AF488); Flow Cytometry

View related series

Click to view target information: HLA-A

Accession#: P01901 (13) AF488 (72) Gene ID: 14972 (17) H-2K (12) H-2K(d) (12) H2-D1 (13) H2-K (12) H2-K1 (17) K-f (12)

Basic Description

Product Name	MHC class I/H-2Kb Mouse mAb (AF488) - Primary antibody, specific to MHC class I/H-2Kb
Synonyms	K-f \| H-2K \| H2-K \| H2-D1 \| H-2K(d) \| A-10 alpha chain \| FLJ26655 \| HLA class I histocompatibility antigen, A-1 alpha chain \| HLA class I histocompatibility antigen, A-28 alpha chain \| HLA class I histocompatibility antigen, A-9 alpha chain \| HLA Class I
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Host species	Mouse
Specificity	MHC class I/H-2Kb
Immunogen	C57BL/6 mouse splenocytes
Positive Control	Flow Cytometry: C57BL/6 mouse splenocytes.
Conjugation	AF488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)	Ex:490nm;Em:525nm
Grade	ExactAb™, Validated
Product Description	Mouse anti Mouse MHC class I/H-2Kb Antibody, Monoclonal (AF6-88.5.5.3), could be used for Flow Cytometry.

Product Properties

Light Chain Type	kappa
Purification Method	Protein G purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296)

MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) - Flow Cytometry
Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) at 0.3μg/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

Flow Cytometry: MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296)

MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) - Flow Cytometry
Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

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