Self-assembled sensing interface based on specific aptamer functionalized Ti3C2 MXene for ultrasensitive antibiotic detection in milk

A self-assembled sensing interface based on Ti 3 C 2 MXene functionalized by aptamer had been successfully constructed in this work, which fully utilizes the electrical properties of Ti 3 C 2 MXene and the specific recognition ability of the aptamer. Subsequently, we studied the performance of the sensing interface using two antibiotic residues as examples. Under the optimal conditions, the sensing interface showed a linear range of 100 fM–1 μM and a limit of detection of 1 fM to detect kanamycin and tetracycline, furthermore the recovery rates were 97.15%–103.00% and 99.559%–106.36%, respectively. In addition, the sensing behaviors of aptamers specific recognition of target can be perceived by the interface, thereby exhibiting different electrical responses. Therefore, the self-assembled sensing interface not only provided a new avenue for the development of ultrasensitive technology in the food analysis field but also for investigating aptamer configurational transformations.