Physicochemical characterization, release profile, and antibacterial mechanisms of caffeic acid phenethyl ester loaded in lipid nanocapsules with lauric acid and glycerol monolaurate

Numerous studies have demonstrated the biological activities of caffeic acid phenethyl ester (CAPE), including antibacterial, antioxidant, and anti-inflammatory properties. However, the application of CAPE is limited by its low bioavailability and stability. In this work, we designed a CAPE loaded nanocapsule system by using polyoxyl-15-hydroxystearate, coconut oil and lecithin (LE)/lauric acid (LA)/glycerol monolaurate (GML) to improve the release rate of CAPE. The Blank-GML-lipid nanocapsules (BK-GML) and CAPE loaded BK-GML (CAPE-GML) were mainly assembled by hydrophobic forces and electrostatic forces, exhibited a typical spherical shape with a diameter size of less than 90 nm. The encapsulation and loading efficiencies of BK-GML and CAPE-GML reached 74.27 % and 9.61 %, respectively. Lipid nanocapsules (LNCs) also demonstrated a good sustained release of CAPE during in vitro stimulated digestion, indicating LNCs enable sustained CAPE release to the colon. Additionally, they showed a strong antibacterial activity against Escherichia coli and Staphylococcus aureus through the damage of the bacterial cytoderm. Also, BK-GML and CAPE-GML could inhibit the contamination and spread of pathogenic bacteria to be further applied in foods and pharmaceuticals industries.