Green extraction for hyperoside from Cuscutae semen by natural deep eutectic solvent

In recent years, natural deep eutectic solvents prepared by natural compounds and natural primary metabolites have been more favored, which are environmentally friendly, low toxicity and easy to biodegrade. In this study, twenty different types of natural deep eutectic solvents (NADES) were prepared by selecting five common hydrogen bond acceptors and nine common hydrogen bond donors using different molar ratios, and then were applied with ultrasound-assisted extraction (UAE) to extract hyperoside, an index component in Cuscutae semen . The results showed that the optimal extraction conditions of hyperoside from the natural deep eutectic solvent prepared by choline chloride and lactic acid (molar ratio 1:2) were as follows: 65℃, 38 min, amount of added water 16 %, liquid to solid ratio 51 mL·g −1 , and the actual yield was 6.766 mg·g −1 . Antioxidant assay proved that NADES extract has antioxidant capacity. The results of this paper show that ultrasound-assisted natural deep eutectic solvent can effectively extract hyperoside from Cuscutae semen , which lays a foundation for the green and efficient extraction of bioactive components in natural plants and in-depth research and development.