Gelling ability and gel structure of soy protein isolate influenced by heating in the presence of various acids

This study investigated the influence of acids on gelation of soy protein isolate (SPI) under heating. Specifically, the SPI solution was acidified to pH 2.5 using hydrochloric acid (HA), acetic acid (AA), tartaric acid (TA) and citric acid (CA), respectively, and then heated at 85 °C. It was found that the SPI gel with the anisotropic structure was formed, which suggested that the gel resulted from the organized arrangement of the SPI fibril. Subsequently, it was confirmed by several techniques such as atomic force microscopy that the above acidic-thermal treatment induced fibrillation of SPI. Furthermore, the ability of these acids to promote gelation and fibrillation of SPI was in the order of CA > TA > HA > AA. In summary, these results suggested that heating at pH 2.5 resulted in SPI fibrillation, thus promoting SPI gelation, and the acidity regulator type had a profound effect.