Flexible chitosan-graphene oxide-based biomacroporous cryogel via grafting cryopolymerization for selective recovery of myricetin from food sample

Myricetin has a significant role in pharmacology, specifically in traditional Chinese medicine. The most intriguing pharmacological action of myricetin consists of its multi-pathway anticancer effects. Therefore, rapid and selective isolation of myricetin from garlic and apple juices has notable pharmacological benefits. Chitosan-graphene oxide-based biomacroporous cryogel are highly efficient and environmentally friendly materials for adsorption. The current study involved the synthesis of chitosan-graphene oxide-based biomacroporous cryogel sorbent for the selective recovery of myricetin. The characterization investigation utilizing SEM, XRD, EDS and FTIR demonstrated the successful synthesis of a chitosan-graphene oxide-based biomacroporous cryogel. Additionally, XPS effectively revealed the interaction of functional groups on the biomacroporous cryogel sorbent with myricetin. The maximum adsorption capacity for myricetin, under optimized conditions, was 167 mg g −1 in 60 min. The equilibrium adsorption capacity was achieved at neutral pH with optimal parameters, and the Freundlich isotherm model was followed. The pseudo-second-order model presented better descriptions of the adsorption process. In addition, the chitosan-graphene oxide-based biomacroporous cryogel exhibited remarkable selectivity: due to involvement of two primary factors: the number of hydroxyl-groups and the fact that the extra hydroxyl group at position 5′ in ring B allows for a more noticeable transfer of the unshared electron pair from oxygen to the π system of the B ring. The adsorption of myricetin decreases to 2 % after three consecutive cycles, indicating the significant reusability of chitosan-graphene oxide-based biomacroporous cryogel. Additionally, the chitosan-graphene oxide based-biomacroporous cryogel that was produced has been utilized after one month and shown a comprehensive shelf-life. Typical adsorption mechanisms include hydrogen bonding and demonstrates significant potential for the efficient and specific isolation of myricetin in complex mixtures. Furthermore, the chitosan-graphene oxide-based biomacroporous cryogel demonstrated a significant rise in recovery of myricetin flavonoid in real samples of garlic and apple juices, rising from 99.66 % to 114.29 %.