Enzyme-Responsive 3D Porous Nanofiber Sponge for Antibiotic-Free Wound Healing

Drug-resistant bacterial infections induced by overuse of antibiotics have become a serious problem threatening human health, and the traditional drug delivery method of integrating drugs into solid polymers suffers from the problem of sustained drug release without distinction, thus accelerating the growth of drug-resistant bacteria. In this paper, a three-dimensional porous fiber sponge (3D-GHT) with controlled release of tannic acid (TA) was developed, which has higher porosity, water absorption, water retention, and hemostatic capacity compared to 2D fiber membranes. The controlled release of TA is achieved by hydrolysis of hyaluronic acid (HA) by hyaluronidase (HAase) released by bacteria. The 3D-GHT exhibits high antimicrobial and antioxidant capacity, good biocompatibility without loading antibiotics. The results show the drug release of 3D-GHT in vitro was significantly increased under the action of HAase, and the antioxidant activity was increased by 26.91%. In vivo experiments show that 3D-GHT can effectively promote wound healing, indicating that 3D-GHT sponge as a wound dressing has great potential application in clinic.