Electrochemiluminescence immunosensor using a lanthanum-based metal-organic framework as signal probe and Cu2MoS4 as a co-reaction promoter for the sensitive detection of anti-Müllerian hormone

In this study, we used meso-tetra (4-carboxyphenyl) porphyrin (TCPP) as an organic ligand to modify a lanthanide-based metal-organic framework as an electrochemiluminescence (ECL) platform to sensitively detect anti-Müllerian hormone (AMH). La-MOF amplified the ECL signal by suppressing the aggregation-caused quenching created by TCPP self-aggregation. Utilizing the reversible cycling of the mixed-valence transition metal ion (Cu + /Cu 2+ and Mo 4+ /Mo 6+ ) and the electrical conductivity of Cu 2 MoS 4 and silver nanoparticle (AgNP), Cu 2 MoS 4 –AgNP as a dual co-reaction promoter constantly generated sulfate radical anions (SO 4 •– ) and thus amplified the ECL signal. Based on this strategy, we built a sandwich-type ECL immunosensor for the sensitive detection of AMH with a linear range of 1 × 10 −4 to 50 ng/mL and a limit of detection of 24 fg/mL. This study provides a novel approach for the sensitive and selective detection of AMH, demonstrating its high potential and practical value for the sensitive detection of AMH.