Determination of doxorubicin hydrochloride using eosin Y as a probe by resonance Rayleigh scattering technique

This study used the acidic anionic dye eosin Y (EY) as a resonance Rayleigh scattering (RRS) probe to develop a method for the detection of doxorubicin hydrochloride (DOX). In dilute hydrochloric acid solution medium, eosin Y interacted with each other through hydrogen bonding to form supramolecular aggregates. The addition of doxorubicin hydrochloride induced an ion-conjugation process and dissociated the hydrogen-bonded supramolecular aggregates that eosin Y had formed. As a result, the RRS signal of eosin Y at 534 nm was significantly diminished. The reaction conditions were optimized and the mechanism of RRS intensity reduction was explored. It was found that the main reason for the weakening of the RRS intensity was the decrease in particle size. The range of 0.5–5.0 mg/L is well-represented by the concentration of doxorubicin hydrochloride, which shows decent linear relationship. The linear regression equation was ΔI = 15044c + 10465 with a correlation coefficient R of 0.9995, and a limit of detection of 0.085 mg/L. The recoveries ranged from 97.33% to 102.94%. This work provided a new, sensitive, and cost-effective method for estimating doxorubicin hydrochloride, which can be successfully applied to the estimation of doxorubicin hydrochloride for injection.