Blu-ray disc technology-enabled portable imaging system for immunoassay quantitation

In this work, we have developed a portable laser scanning imaging system derived from Blu-ray disc technology for high-throughput biochip and sensor array characterization. This portable imaging system was constructed by adapting a standard Blu-ray drive to perform the scanning or imaging of an entire disc or interested regions. In addition, we have compiled a complete operating software program to process the raw data, generate the assay image, and perform quantitative analysis. Furthermore, the developed BD-LSI system was successfully applied to the quantitative detection of Anti-Müllerian hormone (AMH), which is an important biomarker of reproductive potential, in standard physiological buffers and serum samples. The achieved detection limit is as low as 18 pg/mL with a dynamic range of up to 25 ng/mL, which meets the needs of clinical diagnosis. As validated by comparing with the Clinical Laboratory Improvement Amendments (CLIA) data on the same set of samples, the consistent results confirm that this BD-LSI system holds promise for multiplex on-site quantitative analysis at community clinics and even at home settings for self-monitoring of individual health.