Baseline Susceptibility of Plutella xylostella and Spodoptera exigua to Fluxametamide in China

Simple SummaryManagingPlutella xylostellaandSpodoptera exiguahas traditionally relied on chemical insecticides, but resistance to these chemicals has become a major challenge in controlling these pests. Introducing new insecticides with a different mode of action is crucial for effective resistance management. Our study shows that fluxametamide exhibits strong insecticidal activity againstP. xylostellaandS. exigua. We assessed the susceptibility of field populations from key vegetable-growing regions in China and established a baseline of susceptibility to fluxametamide. The findings indicated thatP. xylostellaandS. exiguaare highly susceptible to this insecticide, and the baseline susceptibility data will serve as a reference for future resistance monitoring inP. xylostellaandS. exiguamanagement.Fluxametamide, an innovative isoxazoline insecticide, acts as an antagonist of γ-aminobutyric acid-gated chloride channels. Its distinct mode of action sets it apart, lacking known cross-resistance with current insecticides. This positions fluxametamide as a promising tool for addressing insecticide resistance in Lepidoptera, thysanoptera, coleoptera, and diptera pest insects. To develop and implement successful resistance management strategies, it is crucial to establish the baseline susceptibility to this insecticide before it is registered and widely used in China. In this study, we assessed the baseline susceptibility of two widespread lepidopteran pest species,Plutella xylostellaandSpodoptera exigua, to fluxametamide. The insecticide exhibited remarkably high efficacy against populations of the two lepidopteran species sampled in their primary distribution areas in China. ForP. xylostellaandS. exigua, the median lethal concentrations (LC50) ranged between 0.040 and 0.247 mg/L, and 0.211 and 0.761 mg/L, respectively. Among populations, there was a relative low variability in susceptibility to fluxametamide, showing a 6.18-fold difference forP. xylostellaand 3.61-fold forS. exigua. The suggested diagnostic concentrations forP. xylostellaandS. exiguawere 10 and 15 mg/L, respectively. Fluxametamide exhibited high toxicity to the selected resistant strains, which displayed strong resistance to abamectin, emamectin benzoate, and deltamethrin. No cross-resistance to fluxametamide was detected in the laboratory diamide-resistant strain. Our findings offer essential insights for crafting successful resistance management initiatives to maintain the effectiveness of fluxametamide against these significant pests.