An integrated colorimetric biosensing platform containing microneedle patches and aptasensor for histamine monitoring in seafood

Excessive histamine in spoiled seafood poses considerable health hazards to consumers, yet its detection is challenging due to complicated sample preparation and detection methodologies. Herein, an integrated colorimetric platform containing Poly (vinyl alcohol) (PVA)/ hyaluronic acid (HA) microneedle patchs-assisted extraction and aptasensor-based detection was reported. The developed PVA/HA microneedle patches facilitated on-site histamine extraction from seafood through a two-minute press-and-peel procedure. To enhance detection efficacy, strategies for generating high-affinity aptamers with specific terminal-fixed structures and constructing AuNPs@FeP-chitosan oligosaccharide (COS) nanozyme boosting catalytic efficiency were proposed. Utilizing the aptamer HIS3-T2 in conjunction with the nanozyme, a colorimetric aptasensor was developed. Integrated with the patches, the aptasensor achieved high sensitivity and selectivity, detecting histamine within a range of 2~800   nM with a limit of detection (LOD) of 1.89   nM. Validated on real-world salmon and shrimp samples, this integrated system promises rapid and accurate histamine monitoring, offering great reference for similar applications in food quality control.