Mesoporous MnFe2O4 magnetic nanoparticles as a peroxidase mimic for the colorimetric detection of urine glucose

Mesoporous MnFe2O4 magnetic nanoparticles (mMnFe2O4 MNPs) were prepared with a one-step synthesis method and characterized to possess intrinsic peroxidase-like activity, and had obvious advantages over other peroxidase nanozymes in terms of high catalytic affinity, high stability, mono-dispersion, easy preparation, and quick separation. The mMnFe2O4 MNPs were used as a colorimetric sensor for indirect sensing of urine glucose based on the sensing principle that H2O2 can be produced from glucose oxidation catalyzed by glucose oxidase (GOx), and under the catalysis of the mMnFe2O4 MNPs nanozyme, H2O2 can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue color in a few minutes. This sensor is simple, cheap, sensitive, and specific to glucose detection with a detection limit of 0.7 μM, suggesting its potential for on-site glucose detection.