Adsorption and convenient ELISA detection of sulfamethazine in milk based on MOFs pretreatment

Metal-organic frameworks (MOFs) has excellent adsorption performance, herein, three kinds of common MOFs were used for the adsorption of sulfamethazine (SM 2 ) in milk, then enzyme-linked immunosorbent assay (MOF-ELISA) was established. Firstly, NH 2 -UiO-66, NH 2 -MIL-101, and ZIF-8 were successfully prepared and their adsorption characteristics for SM 2 were investigated. The kinetic models of the three MOFs were more in line with the pseudo-second-order adsorption kinetics, and the saturated adsorption capacity of NH 2 -UiO-66, NH 2 -MIL-101, and ZIF-8 for SM 2 at 298 K were 139.64, 29.98, and 36.5 mg/g, respectively. Using three different MOFs as adsorbents, the pretreatment of milk samples could be completed within 1 h, the half inhibitory concentrations (IC 50 ) of MOF-ELISA were 1.26, 1.86 and 2.74 ng/mL, the limit of detections (LOD) were 0.05, 0.12, and 0.19 ng/mL and the recovery rate were from 82.30% to 105.62% with the intra-day coefficient of variations (CVs) below 5.81% and inter-day CVs below 7.21%. Detection results showed good correlations with LC-MS/MS (R 2  > 0.99), indicated that MOFs could effectively eliminate the interference of sample matrix, and has the potential to become a general pretreatment method for the detection of various matrices residues in food safety monitoring.