A multifunctional chitosan hydrogel dressing for liver hemostasis and infected wound healing

For the treatment of infected bleeding wounds, we compounded methacrylate anhydride dopamine (DAMA) and Zn-doped whitlockite nanoparticles (Zn-nWH) into methacrylate anhydride quaternized chitosan (QCSMA) to obtain a multifunctional hydrogel dressing (QCSMA/DAMA/Zn-nWH) with hemostasis , disinfection and wound healing promotion. QCSMA/DAMA/Zn-nWH exhibited good adhesion (0.031 MPa) and DPPH scavenging ability (94%), favorable biocompatibility (hemolysis ratio < 2%, no cytotoxicity), and showed a low BCI value (< 13%) in vitro coagulation test and could activate coagulation pathway. In addition, QCSMA/DAMA/Zn-nWH had excellent hemostatic effect (129 ± 22 s, 27 ± 5 mg) in vivo compared with the control (571 ± 15 s, 147 ± 31 mg) and CCS (354 ± 27 s, 110 ± 46 mg). Meanwhile, QCSMA/DAMA/Zn-nWH showed excellent antibacterial properties (> 90% against S. aureus and E. coli ) and could promote collagen deposition, reduce inflammatory expression and promote wound healing. All results indicate that these multifunctional hydrogel dressings have great potential in clinical hemostasis and anti-infection healing.