Recombinant protein G/Au nanoparticles/graphene oxide modified electrodes used as an electrochemical biosensor for Brucella Testing in milk

In this study, a simple label-free biosensor for Brucella was constructed, which based on the screen-printed carbon electrode (SPCE) modified by Recombinant protein G/gold nanoparticles/graphene oxide (RpG/Au/GO). The impedance responses of the proposed biosensor were measured by electrochemical AC impedance method in Brucella antigen gradient concentration solutions. The results showed that the linear range of this biosensor was from 1.6 × 10 2  CFU/mL to 1.6 × 10 8  CFU/mL with the minimum detection limit of 3.2 × 10 2  CFU/mL (S/N = 3). Moreover, the biosensor for Brucella detection possessed acceptable reproducibility with a relative standard deviation of 5.15% and acceptable stability with a relative standard deviation of 4.68%. The spiked recovery rate in actual pasteurized milk samples was more than 92% . Therefore, the developed biosensor exhibits excellent prospects in the selective quantification detection of Brucella abortus .