High efficient electrochemical biosensor based on exonuclease-Ⅲ-assisted dual-recycling amplification for ultrasensitive detection of kanamycin

A target-triggered and exonuclease-Ⅲ-assisted strand displacement, dual-recycling amplification reaction-based biosensor was developed for the rapid, ultrasensitive and accurate detection of kanamycin . The robust profiling platform was constructed using high conductive MXene/VS 2 for the electrode surface modification and high active CeCu 2 O 4 bimetallic nanoparticles as nanozyme to improve the sensitivity as well as the catalytic signal amplification of the biosensor. Using the dual supplementary recycling of primer DNA and hairpin DNA , the electrochemical platform could accurately detect kanamycin to as low as 0.6 pM from the range of 5 pM to 5 μM. By profiling five other antibiotics, this platform exhibited high specificity, enhanced repeatability and reproducibility. Based on these intrinsic characteristics and by utilizing milk and water samples, the as-designed biosensor offers a remarkable strategy for antibiotic detection due to its favorable analytical accuracy and reliability, thereby demonstrating potential application prospect for various antibiotic biosensing in food quality control, water contamination detection and biological safety analysis.