Detection of Glioma Cells based on Electrochemical Sensor Based on an Aptamer Method Recognition

The detection of glioma cells (GMC) could be used to evaluate the effectiveness of treatment. Based on the advantages of easy synthesis and modification of aptamers and the advantages of high sensitivity, excellent selectivity, and wide application of electrochemical methods, this work constructs an aptamer electrochemical sensor that can be used for rapid and efficient detection of GMC. We used activators for the activation of carboxyl groups after modification of 4-carboxyphenyl diazonium salt. The aptamer, which can specifically recognize GMC, is immobilized on the electrode surface by forming an amide bond between the amino and carboxyl groups on the aptamer. The conditions affecting the sensing of the aptamer sensor were investigated in detail. The current of the aptamer sensor is proportionally with the GMC concentration from 1×10 7 to 1×10 2 cells/mL. Moreover, the sensor can identify GMC with reasonable specificity.