Novel Glucose Oxidase Interlocked Prussian Blue/Polysulfone Stereo-Structure and its Application in Amperometric Glucose Biosensor

In this study, we present a controllable Prussian Blue (PB)- Polysulfone (PSF) stereo-structure with glucose oxidase (GOD) interlocked for glucose biosensing. This work links the electrochemical catalytic properties of PB with the large surface area and mass transport of three-dimensional macro-porous PSF and highlights the importance of interlocking GOD with PB to improve sensor sensitivity and linearity, which also maintains glucose sensor activity. The prepared PB-PSF-GOD glucose biosensor exhibited a 1-12 mM linear range, high affinity and good selectivity with a low applied potential of -0.05 V (vs Ag/AgCl). Furthermore, the prepared sensor only attenuated by 10% after 7 days of continuous testing. The advanced properties of the novel GOD interlocked PB-PSF structure may have promising applications in enzymatic biosensors, especially the continuous glucose monitoring (CGM).