Molecularly imprinted electrochemical sensor based on metal-covalent organic framework for specifically recognizing norfloxacin from unpretreated milk

Here, a molecularly imprinted electrochemical sensor (MIECS) was designed and fabricated for specifically monitoring norfloxacin (NFX), an entirely synthetic antibiotic. In which, Cu 2+ dopped covalent organic framework (COF) was used to connect NFX imprinting layer and glassy carbon electrode through covalence. Under optimized conditions, the linear range is as wide as 5 orders of magnitude, and the detection limit is 5.94 × 10 −3 μM (estimated based on S/N = 3). Average recoveries are among 92.4%–99.0% with relative standard deviations ≤ 4.05% (n = 3) in (spiked) whole, low-fat, and skimmed milk, validated by independent HPLC assays. The excellent performance can be ascribed to the significant recognition and enriching ability of the imprinting layer, improved conductivity of Cu 2+ dopped covalent organic framework, and high stability of covalence between layers. We hope the work will act as a model of MIECSs for rapidly and selectively detecting trace drug residue in complex real samples.