Electrochemical sensitive detection of cannabidiol based on carbon black/gold nanoparticle composite modified glassy carbon electrode

Cannabidiol (CBD), a significant secondary metabolite of Cannabis sativa L. , has pharmacological effects for the treatment of a variety of health conditions, including nerve protection, epilepsy, anti-inflammatory, anti-anxiety, and cancer, which have garnered increasing interest in recent years. Herein, an electrochemical sensing platform was constructed for ultrasensitive determination of CBD, on the base of a glassy carbon electrode (GCE) chemically modified with carbon black (CB) and gold nanoparticles (AuNPs) (CB/AuNPs/GCE). The morphology, structure and electrochemical performance of prepared CB/AuNPs/GCE were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), Ultraviolet-visible spectroscopy (UV–vis), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and electrochemical technologies. The effects of pH, scanning rate, enrichment time, enrichment potential and the speculated mechanism of cannabidiol reaction at modified electrode were studied in detail. Under the optimized conditions, the fabricated sensing platform exhibited a great linear response of 0.42 μA μM −1 in the concentration range of 0.25 ∼ 50.0 μM. The prepared CB/AuNPs/GCE showed long-term stability, good repeatability and high practicability in real samples of industrial hemp. The proposed sensor can be further enhanced for portable and rapid detection of plant secondary metabolites, providing a basis for food detection, agricultural development, and drug detection.