Biocatalytic membranes with crosslinked enzyme aggregates for micropollutant removal

he removal of micropollutants has drawn increasing attention due to the serious hazard even at low concentration. The construction of separation membranes with high micropollutant removal is highly desired. In this study, laccase cross-linked enzyme aggregates (CLEAs) were obtained with bovine serum albumin as a protective agent, followed by the preparation of biocatalytic membrane using glutaraldehyde as a crosslinking agent and preloaded polydopamine-modified UiO-66-NH 2 as the intermediate layer. The membrane was systematically characterized. Under the optimal preparation conditions, the bisphenol A removal rate was 97.13% with high applicability at different feed pH and feed concentration with good storage stability and reusability. The multifunctional synergistic effect of the biocatalytic membrane for micropollutant removal by rejection, adsorption and catalysis was analyzed. The membrane also has good treatment effect on micropollutants like antibiotics and for dye/salt mixture solution. Besides, the preparation method was expanded by replacing laccase with phosphotriesterase (PTE) to fabricate PTE-CLEAs biocatalytic membrane, which can have high removal rate of 94.04% for organophosphorus pesticide methyl paraoxophosphorus in water.