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LightNing™ BsaI

COA

Grade & Purity:

for molecular biology

for DNA and RNA applications

20 U/μl

In stock

Item Number

L359050

Grouped product items
SKU	Size	Availability	Price	Qty
L359050-1KU	1KU	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$39.90
L359050-5KU	5KU	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$149.90

View related series

Endonuclease (5) Nuclease (7)

Basic Description

Synonyms

Eco31I | BsaI

Specifications & Purity

BioReagent, PCR Reagent, for DNA synthesis, Suitable for molecular biology, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, 20 U/μl

Stability And Storage

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Storage Temp

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for DNA and RNA applications, for DNA synthesis, for molecular biology, PCR Reagent, 废弃Ph. Eur., EnzymoPure™

Product Description

Restriction enzymes are a class of endonuclease enzymes that recognize specific sequences of deoxyribonucleotides and cut the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI is an IIS restriction enzyme that recognizes non-palindromic sequences and cuts sticky ends outside the recognition sequence.

Source
E.coli

Restriction Enzyme CuttingSite
5’…GGTCTC(N)1↓…3’
3’…CCAGAG(N)5↑…5’

Enzyme Activity Definition
The amount of enzyme required to fully digest 1 μg λDNA Dcm-HindIII fragments within 1 h in a 50 μl reaction system at 37℃ is defined as 1 activity unit (U).

Protocol
Example 1: DNA rapid enzyme digestion
(1) Configure the mixture as shown in the following table:

Component	Genome DNA	PCR Product	Plasmid DNA
RNase-free Water	To 50 μl	To 30 μl	To 20 μl
10× Cut Reaction Buffer	5 μl	3 μl	2 μl
DNA	10 μl (5 μg)	10 μl (0.2 μg)	2 μl (1 μg)
BsaI	5 μl	1 μl	1 μl

(2) Gently mix, do not oscillate the vortex, and then instantaneous centrifugation.
(3) Reaction at 37℃ for 15 min (plasmid), or 15-30min (PCR product), or 30-60 min (genomic DNA).
(4) Reaction at 80℃ for 20 min can deactivate the enzyme and terminate the reaction (optional).

Example 2: It is suitable for extended reaction system of plasmids

RNase-free Water	To 20 μl	To 20 μl	To 30 μl	To 40 μl	To 50 μl
10× Cut Reaction Buffer	2μl	2 μl	3 μl	4 μl	5 μl
Plasmid	1 μg	2 μg	3 μg	4 μg	5 μg
BsaI	1 μl	2 μl	3 μl	4 μl	5 μl

Cautions
(1) The methylation of Dcm, CpG and EcoB I will impair the cutting activity of this product.
(2) Unpurified PCR products have a certain ionic strength. In addition, since DNA polymerase also has exonuclidene activity, it will affect the enzyme digestion products. Therefore, cloning and other operations need to be performed in the following step.
(3) This product is for scientific research only and shall not be used for other purposes

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

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