Skip to the beginning of the images gallery

LFM-A13 - ≥98%(HPLC), high purity , CAS No.62004-35-7

COA

Grade & Purity:

≥98%(HPLC)

Cas Number: 62004-35-7
Molecular Weight: 360
MDL number: MFCD02179204
PubChem CID: 54686938
PubChem SID: 488201704

In stock

Item Number

L286934

Grouped product items
SKU	Size	Availability	Price
L286934-5mg	5mg	3	$124.90
L286934-10mg	10mg	3	$191.90
L286934-25mg	25mg	3	$431.90
L286934-50mg	50mg	1	$670.90
L286934-100mg	100mg	2	$1,207.90
L286934-250mg	250mg	2	$2,716.90

Potent, selective BTK inhibitor

View related series

Cell Cycle (2830) Cell signal transduction and Neuroscience (85) Histone Modification (1379) Non-receptor Tyrosine Kinase (643)

Basic Description

Synonyms	AKOS028108222 \| alpha-Cyano-beta-hydroxy-beta-methyl-N-(2,5-dibromophenyl)propenamide \| KBio3_001038 \| HMS3370M16 \| L0309 \| 2-Cyano-N-(2,5-dibromophenyl)-3-hydroxy-2-butenamide \| Bio2_000450 \| KBio2_000579 \| KBio2_005715 \| KBio3_001037 \| Q27166836 \| KBioG
Specifications & Purity	≥98%(HPLC)
Biochemical and Physiological Mechanisms	Potent and selective inhibitor of Bruton's tyrosine kinase (BTK). Inhibits recombinant BTK with an IC50value of 2.5μM and has no activity on other protein kinases ( including JAK1, JAK3, HCK, EGFR kinase and insulin receptor kinase) at concentrations of u
Storage Temp	Protected from light,Store at -20°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	Product description： A potent, cell-permeable, reversible, substrate competitive, and specific inhibitor of Bruton’s Tyrosine Kinase (BTK; IC50 = 17.2 µM for human BTK in vitro and IC50 = 2.5 µM for recombinant BTK). Also inhibits Polo-like kinase in an ATP-competitive manner (IC50 = 10 µM and 61 µM for Plx1 and Plk3, respectively), and displays antitumor properties. Does not affect the enzymatic activity of other protein tyrosine kinases, including EGFR, HCK, IRK JAK1, and JAK3 even at concentrations of 278 µM. Enhances the chemosensitivity of BTK-positive B-lineage leukemia cells to ceramide- and vincristine-induced apoptosis. A potent, cell-permeable, reversible, substrate competitive, and specific inhibitor of Bruton′s tyrosine kinase (BTK; IC50 = 17.2 µM for human BTK in vitro and IC50 = 2.5 µM for recombinant BTK). Also inhibits Polo-like kinase in an ATP-competitive manner (IC50 = 10 µM and 61 µM for Plx1 and Plk3, respectively), and displays antitumor properties. Does not affect the enzymatic activity of other protein tyrosine kinases, including EGFR, HCK, IRK JAK1, and JAK3 even at concentrations of 278 µM. Enhances the chemosensitivity of BTK-positive B-lineage leukemia cells to ceramide- and vincristine-induced apoptosis.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Benzenoids
  - Class Benzene and substituted derivatives
    - Subclass Anilides

Kingdom	Organic compounds
Superclass	Benzenoids
Class	Benzene and substituted derivatives
Subclass	Anilides
Intermediate Tree Nodes	Not available
Direct Parent	Anilides
Alternative Parents	N-arylamides Bromobenzenes Aryl bromides Vinylogous acids Secondary carboxylic acid amides Nitriles Enols Organopnictogen compounds Organobromides Organic oxides Hydrocarbon derivatives Carbonyl compounds
Molecular Framework	Aromatic homomonocyclic compounds
Substituents	Anilide - N-arylamide - Bromobenzene - Halobenzene - Aryl bromide - Aryl halide - Vinylogous acid - Carboxamide group - Secondary carboxylic acid amide - Carboxylic acid derivative - Enol - Carbonitrile - Nitrile - Organopnictogen compound - Organic oxygen compound - Hydrocarbon derivative - Organic nitrogen compound - Organic oxide - Carbonyl group - Organohalogen compound - Organobromide - Organonitrogen compound - Organooxygen compound - Aromatic homomonocyclic compound
Description	This compound belongs to the class of organic compounds known as anilides. These are organic heterocyclic compounds derived from oxoacids RkE(=O)l(OH)m (l not 0) by replacing an OH group by the NHPh group or derivative formed by ring substitution.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Names and Identifiers

Pubchem Sid	488201704
Pubchem Sid Url	https://pubchem.ncbi.nlm.nih.gov/substance/488201704
IUPAC Name	2-cyano-N-(2,5-dibromophenyl)-3-hydroxybut-2-enamide
INCHI	InChI=1S/C11H8Br2N2O2/c1-6(16)8(5-14)11(17)15-10-4-7(12)2-3-9(10)13/h2-4,16H,1H3,(H,15,17)
InChIKey	UVSVTDVJQAJIFG-UHFFFAOYSA-N
Smiles	CC(=C(C#N)C(=O)NC1=C(C=CC(=C1)Br)Br)O
Isomeric SMILES	CC(=C(C#N)C(=O)NC1=C(C=CC(=C1)Br)Br)O
Molecular Weight	360
Reaxy-Rn	10678941
Reaxys-RN_link_address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=10678941&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

9 results found

Lot Number	Certificate Type	Date	Item
K2225712	Certificate of Analysis	Aug 15, 2022	L286934
C2519733	Certificate of Analysis	Aug 15, 2022	L286934
K2225676	Certificate of Analysis	Aug 15, 2022	L286934
K2225677	Certificate of Analysis	Aug 15, 2022	L286934
K2225604	Certificate of Analysis	Aug 15, 2022	L286934
K2225780	Certificate of Analysis	Aug 15, 2022	L286934
K2225664	Certificate of Analysis	Aug 15, 2022	L286934
K2225667	Certificate of Analysis	Aug 15, 2022	L286934
K2225779	Certificate of Analysis	Aug 15, 2022	L286934

Chemical and Physical Properties

Solubility	Solvent:DMSO, Max Conc. mg/mL: 36, Max Conc. mM: 100
Sensitivity	light & heat sensitive
Molecular Weight	360.000 g/mol
XLogP3	3.800
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Exact Mass	359.893 Da
Monoisotopic Mass	357.895 Da
Topological Polar Surface Area	73.100 Å²
Heavy Atom Count	17
Formal Charge	0
Complexity	386.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	1
The total count of all stereochemical bonds	1
Covalently-Bonded Unit Count	1

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration