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Basic Description
| Product Name | L-Glutamic Dehydrogenase (NADP) - >60 U/mg, high purity , CAS No.9029-11-2 |
|---|---|
| Synonyms | L-Glutamate:NADP+ oxidoreductase (deaminating) |
| Grade | EnzymoPure™ |
| Product Description |
Application This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis. In vitro, various activity assays of this enzyme examine the conversion of α-ketoglutarate to L-glutamate, in the presence of excess ammonium ions (NH4+) and NADPH. physical property Isoelectric point : 4.6 Michaelis constants : 1.1 X 10-3M (NH3), 3.4 X 10-4M (α-Ketoglutarate) 1.2 X 10-3M (L-Glutamate), 1.4 X 10-5M (NADPH), 1.5 X 10-5M (NADP+) Structure : 6 subunits (M.W.50,000) per mol of enzyme Inhibitors : Hg++, Cd++, p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine, 2,2′-dithiopyridine Optimum pH : 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG) Optimum temperature : 45oC(α-KG−L-Glu) 45-55oC (L-Glu→α-KG) pH stability : pH 6.0 - 8.5 (25oC, 20hr) Thermal stability : below 50oC (pH 7.4, 10min) |
| Specifications & Purity | EnzymoPure™, ＞60 U/mg |
| Source | bacterial |
Storage and Shipping
| Concentration | ＞60 U/mg |
|---|---|
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| CAS | 9029-11-2 |
| Enzyme Commission Number | 1.4.1.4 |
| Unit definition | One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30 °C in the presence of ammonium ions and NADPH. |
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Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 G2402358 |
Certificate of Analysis | Jan 30, 2024 | L489276 |
| G2402359 |
Certificate of Analysis | Jan 30, 2024 | L489276 |
| H2304226 |
Certificate of Analysis | Sep 14, 2022 | L489276 |
| K2204625 |
Certificate of Analysis | Sep 14, 2022 | L489276 |
| L2318024 |
Certificate of Analysis | Sep 14, 2022 | L489276 |
| D2319421 |
Certificate of Analysis | Sep 14, 2022 | L489276 |
| E2315475 |
Certificate of Analysis | Sep 14, 2022 | L489276 |
| J2307022 |
Certificate of Analysis | Sep 14, 2022 | L489276 |
| K2204633 |
Certificate of Analysis | Sep 14, 2022 | L489276 |
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