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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab112385-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab112385-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab112385-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
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Ab112385-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
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mAb (OTI2C1); Mouse anti Human KRAS Antibody; WB, IHC; Unconjugated
| Product Name | KRAS Mouse mAb - Primary antibody, specific to KRAS |
|---|---|
| Synonyms | c Ki ras2 antibody | c Kirsten ras protein antibody | c-K-ras antibody | c-Ki-ras antibody | Cellular c Ki ras2 proto oncogene antibody | Cellular transforming proto oncogene antibody | CFC2 antibody | cK Ras antibody | GTPase KRas antibody | K RAS p21 pr |
| Specifications & Purity | ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| Host species | Mouse |
| Specificity | KRAS |
| Immunogen | Full length recombinant protein of human KRAS. |
| Positive Control | WB: HepG2, HeLa, A549, MDCK, PC12, MCF7, K562, Ramos, 293T, COS7 and Jurkat lysates. IHC: Human normal colon tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human KRAS Antibody, Monoclonal (OTI2C1), could be used for WB, IHC and so on.
|
| SDS-PAGE | 150 kDa |
|---|---|
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
KRAS Mouse mAb (Ab112385) - Western Blot
All lanes: KRAS Mouse mAb (Ab112385) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 21 kDa
Exposure time: 15.0 s
KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemistry analysis of paraffin-embedded human normal colon labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemical staining of paraffin-embedded human normal brain labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemical staining of paraffin-embedded human normal brain labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
KRAS Mouse mAb (Ab112385) - IHC
Immunohistochemical staining of paraffin-embedded human normal kidney labelling KRAS with KRAS Mouse mAb (Ab112385) at 1/100 dilution. The section was incubated with KRAS Mouse mAb (Ab112385) for 1 h at room temperature. Results show nuclear and cytoplasmic staining. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
| Application | Dilution info |
|---|---|
| WB | 1/2000 |
| IHC | 1/100 |