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iNOS Mouse mAb - Primary antibody, specific to Human iNOS

    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: AB01/2H7
  • Conjugation: Unconjugated
In stock
Item Number
Ab169216
Grouped product items
SKU Size
Availability
Price Qty
Ab169216-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab169216-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab169216-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab169216-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb (AB01/2H7); Mouse anti Human iNOS; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated

Basic Description

Product Name iNOS Mouse mAb - Primary antibody, specific to Human iNOS
Synonyms HEP-NOS antibody | Hepatocyte NOS antibody | HEPNOS antibody | inducible antibody | Inducible nitric oxide synthase antibody | Inducible NO synthase antibody | Inducible NOS antibody | iNOS antibody | MAC NOS antibody | Macrophage NOS antibody | Nitric ox
Specifications & Purity High Performance, Azide Free, Animal Free, Carrier Free, Validated, ExactAb™, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic targ
Host species Mouse
Specificity Human iNOS
Immunogen Recombinant protein corresponding to Human iNOS
Positive Control WB: Human serum Flow Cytometry: A549 cells IF/ICC: MCF-7 cells
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human iNOS Antibody, clone AB01/2H7, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

NOS2 Tchem Nitric oxide synthase, inducible (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
NOS2 Tchem Nitric oxide synthase, inducible (1636 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

iNOS Mouse mAb (Ab169216) - Western Blot
All lanes: iNOS Mouse Antibody (Ab169216) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 141 kDa
Exposure time: 30.0 s

iNOS Mouse mAb (Ab169216) - Flow Cytometry 
Flow Cytometry analysis of A549 cells stained with iNOS Mouse mAb (Ab169216) (Red) at 5.0 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).​

iNOS Mouse mAb (Ab169216) - ICC/IF
IF analysis of iNOS (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with iNOS Mouse mAb (Ab169216) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
WB

1/1000 - 1/2000

IF/ICC

1/200

Flow Cytometry

1/200-1/400

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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Customer Reviews

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