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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab169216-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab169216-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$199.90
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Ab169216-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
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Ab169216-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
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mAb (AB01/2H7); Mouse anti Human iNOS; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated
| Product Name | iNOS Mouse mAb - Primary antibody, specific to Human iNOS |
|---|---|
| Synonyms | HEP-NOS antibody | Hepatocyte NOS antibody | HEPNOS antibody | inducible antibody | Inducible nitric oxide synthase antibody | Inducible NO synthase antibody | Inducible NOS antibody | iNOS antibody | MAC NOS antibody | Macrophage NOS antibody | Nitric ox |
| Specifications & Purity | High Performance, Azide Free, Animal Free, Carrier Free, Validated, ExactAb™, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic targ |
| Host species | Mouse |
| Specificity | Human iNOS |
| Immunogen | Recombinant protein corresponding to Human iNOS |
| Positive Control | WB: Human serum Flow Cytometry: A549 cells IF/ICC: MCF-7 cells |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human iNOS Antibody, clone AB01/2H7, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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iNOS Mouse mAb (Ab169216) - Western Blot
All lanes: iNOS Mouse Antibody (Ab169216) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 141 kDa
Exposure time: 30.0 s
iNOS Mouse mAb (Ab169216) - Flow Cytometry
Flow Cytometry analysis of A549 cells stained with iNOS Mouse mAb (Ab169216) (Red) at 5.0 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG1 was used as an isotype control (Blue).
iNOS Mouse mAb (Ab169216) - ICC/IF
IF analysis of iNOS (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with iNOS Mouse mAb (Ab169216) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
| IF/ICC | 1/200 |
| Flow Cytometry |
1/200-1/400
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Starting at $66.90