Hygromycin B from Streptomyces hygroscopicus - powder, BioReagent, suitable for cell culture, suitable for insect cell culture, high purity , CAS No.31282-04-9
Hygromycin B from Streptomyces hygroscopicus - powder, BioReagent, suitable for cell culture, suitable for insect cell culture, high purity , CAS No.31282-04-9
BioReagent, for cell culture, for insect cell culture, powder
Biochemical and Physiological Mechanisms
Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher euk
Storage Temp
Store at 2-8°C
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, for cell culture, for insect cell culture
Note
注意: 潮霉素B产品应在Store at 2-8°C的温度下保存，其干燥固定形式可在Store at 2-8°C的温度下保存至少五年。其在37°C下可稳定30天。 （对于溶液）如果在Store at 2-8°C下保存，该溶液可在两年内保持稳定。
Product Description
Chemical structure: aminoglycoside Aminoglycosides are the most widely used class of antibiotics that target ribosome. Hygromycin B is an aminocyclitol antibiotic. The organism, S. hygroscopicus contains phosphotransferase (HPH) activity, which phosphorylates hygromycin B helping in autoimmunity against the toxic effects of the drug.
Application
Hygromycin B antibiotic was used for hygro resistance effect in the following cells: transfected 293T cells with mixture of plasmids at 160 μg/ml. MCF-7 ALOX12 cells at 150 μg/ml. MSCV Luciferase PGK-hygro plasmid. STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.
This compound belongs to the class of organic compounds known as 2-deoxystreptamine aminoglycosides. These are aminoglycosides containing the 2-deoxystreptamine (1,3-diaminocyclohexane-4,5,6-triol) core.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
2 Citations
G2315532