45 Citations
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Basic Description
| Product Name | Hyaluronidase - ≥ 300 IU/mg, from bovine testes, high purity , CAS No.37259-53-3 |
|---|---|
| Synonyms | Hyaluronate Lyase |
| Grade | EnzymoPure™ |
| Product Description |
Hyaluronidase is widely present in nature.In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards. Application
Hyaluronidase from Streptomyces hyalurolyticus has been used:in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue；as a component of digestion solution for the derivation of germline stem cells from testicular tissue.
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| Specifications & Purity | EnzymoPure™, ≥ 300 IU/mg, from bovine testes |
| Biochemical and Physiological Mechanisms | Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is |
Storage and Shipping
| Concentration | ≥ 300 IU/mg, from bovine testes |
|---|---|
| Storage Temp | Store at -20°C |
| Shipped In | Ice chest + Ice pads |
| CAS | 37259-53-3 |
| Enzyme Commission Number | 3.2.1.35 |
| Unit definition | Under conditions of 37 ℃ and pH 5.3-5.7, one unit can cause a 0.330% change in transmittance of 2.0 mL reaction mixture at 600 nm (detected at 45 min) |
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 F2327336 |
Certificate of Analysis | Apr 18, 2025 | H141272 |
| A2305529 |
Certificate of Analysis | Apr 18, 2025 | H141272 |
| F2327337 |
Certificate of Analysis | Apr 09, 2025 | H141272 |
| A2305553 |
Certificate of Analysis | Oct 17, 2024 | H141272 |
| A2305545 |
Certificate of Analysis | Oct 17, 2024 | H141272 |
| J2418477 |
Certificate of Analysis | Oct 14, 2024 | H141272 |
| J2418483 |
Certificate of Analysis | Oct 14, 2024 | H141272 |
| J2418474 |
Certificate of Analysis | Oct 14, 2024 | H141272 |
| I2406314 |
Certificate of Analysis | Aug 29, 2024 | H141272 |
| I2406315 |
Certificate of Analysis | Aug 29, 2024 | H141272 |
| I2406320 |
Certificate of Analysis | Aug 29, 2024 | H141272 |
| I2226302 |
Certificate of Analysis | Jun 17, 2024 | H141272 |
| G2229349 |
Certificate of Analysis | May 15, 2024 | H141272 |
| F2327347 |
Certificate of Analysis | Jun 16, 2023 | H141272 |
| A2305544 |
Certificate of Analysis | Nov 24, 2022 | H141272 |
| A2305552 |
Certificate of Analysis | Nov 24, 2022 | H141272 |
| I2226303 |
Certificate of Analysis | Sep 16, 2022 | H141272 |
| I2226304 |
Certificate of Analysis | Sep 16, 2022 | H141272 |
| G2229358 |
Certificate of Analysis | Jul 21, 2022 | H141272 |
| G2229395 |
Certificate of Analysis | Jul 21, 2022 | H141272 |
| F2201131 |
Certificate of Analysis | Mar 18, 2022 | H141272 |
| F2201134 |
Certificate of Analysis | Mar 18, 2022 | H141272 |
| F2201129 |
Certificate of Analysis | Mar 18, 2022 | H141272 |
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Documents & Articles
Hyaluronan
Categories: Technical articles
Citations of This Product
| 1. Junkai Jiang, Jialiang Xie, Liang Zhou, Wei Han, Jing Ye, Daorun Hu, Weichang Xie, Jianbin Qiu, Runfu Chen, Xiaolei Wang. (2023) Near infrared responsive nitric oxide and carbon monoxide nanoplatform for synergistic photodynamic therapy against periodontitis. CHEMICAL ENGINEERING JOURNAL, (147850). |
| 2. Shaowei Pan, Zhaowei Yin, Chen Shi, Haonan Xiu, Guanfu Wu, Yongyuan Heng, Zhangyu Zhu, Jing Zhang, Jianchao Gui, Ziyi Yu, Bin Liang. (2023) Multifunctional Injectable Hydrogel Microparticles Loaded with miR-29a Abundant BMSCs Derived Exosomes Enhanced Bone Regeneration by Regulating Osteogenesis and Angiogenesis. Small, (2306721). |
| 3. Zhijun Zhang, Tiehong Yang, Jingwei Wang, Zhe Yu, Youbei Qiao, Chaoli Wang, Zhenggang Yue, Hong Wu. (2023) Hollow Mesoporous Molybdenum Single-Atom Nanozyme-Based Reactor for Enhanced Cascade Catalytic Antibacterial Therapy. International Journal of Nanomedicine, |
| 4. Zhaowei Yin, Chaoren Qin, Shaowei Pan, Chen Shi, Guanfu Wu, Yan Feng, Jing Zhang, Ziyi Yu, Bin Liang, Jianchao Gui. (2023) Injectable hyperbranched PEG crosslinked hyaluronan hydrogel microparticles containing mir-99a-3p modified subcutaneous ADSCs-derived exosomes was beneficial for long-term treatment of osteoarthritis. Materials Today Bio, 23 (100813). |
| 5. Pan Zejun, Hou Mingzhuang, Zhang Yijian, Liu Yang, Tian Xin, Hu Xiayu, Ge Xiaoyang, Zhao Zhijian, Liu Tao, Xu Yong, Yang Huilin, Liu Hao, Zhu Xuesong, He Fan. (2023) Incorporation of kartogenin and silk fibroin scaffolds promotes rat articular cartilage regeneration through enhancement of antioxidant functions. Regenerative Biomaterials, |
| 6. Songchao Duan, Fangfang Sun, Pan Qiao, Zhihui Zhu, Meilin Geng, Xiaobao Gong, Ying Li, Hanchun Yao. (2023) Detachable Dual-Targeting Nanoparticles for Improving the Antitumor Effect by Extracellular Matrix Depletion. ACS Biomaterials Science & Engineering, 9 (3): (1437–1449). |
| 7. Qiannan Li, Yufei Wang, Qianqian Zhu, Haifang Liu, Jianbo Liu, Hong-Min Meng, Zhaohui Li. (2023) A dual-mode system based on molybdophosphoric heteropoly acid and fluorescent microspheres for the reliable and ultrasensitive detection of alkaline phosphatase. ANALYST, 148 (6): (1259-1264). |
| 8. Chen Zhang, Zhengxiang Cheng, Yuanyuan Zhou, Ziyi Yu, Hongyu Mai, Changhao Xu, Jing Zhang, Jiali Wang. (2022) The novel hyaluronic acid granular hydrogel attenuates osteoarthritis progression by inhibiting the TLR-2/NF-κB signaling pathway through suppressing cellular senescence. Bioengineering & Translational Medicine, 8 (3): (e10475). |
| 9. Mengru Chen, Jiaqi Lu, Jun Hou, Yanbao Zhao, Lei Sun. (2022) Honeycomb-like porous silica nanoparticles for photo and chemo combination therapy. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 77 (103897). |
| 10. Benbo Zhao, Mingda Zhao, Liming Li, Shixiong Sun, Heping Yu, Yuan Cheng, Yuedi Yang, Yujiang Fan, Yong Sun. (2022) Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (17): (9962). |
| 11. Sirui Han, Min Fan, Guichan Xu, Wenting Liu, Jingbo Chen, Yudong Lu, Shangyuan Feng, Ruiyun You. (2022) Gold-Nanostar-Based Surface-Enhanced Raman Scattering Substrates for Gastric Carcinoma Detection. ACS Applied Nano Materials, 5 (9): (12607–12615). |
| 12. Siqi Ma, Zhaoqing Cong, Jiaxing Wei, Weiya Chen, Di Ge, Feifei Yang, Yonghong Liao. (2022) Pulmonary delivery of size-transformable nanoparticles improves tumor accumulation and penetration for chemo-sonodynamic combination therapy. JOURNAL OF CONTROLLED RELEASE, 350 (132). |
| 13. Zhao Xintong, Hua Yujie, Wang Tao, Ci Zheng, Zhang Yixin, Wang Xiaoyun, Lin Qiuning, Zhu Linyong, Zhou Guangdong. (2022) In vitro Cartilage Regeneration Regulated by a Hydrostatic Pressure Bioreactor Based on Hybrid Photocrosslinkable Hydrogels. Frontiers in Bioengineering and Biotechnology, 10 |
| 14. Bin Liu, Yulong Bian, Shuang Liang, Meng Yuan, Shuming Dong, Fei He, Shili Gai, Piaoping Yang, Ziyong Cheng, Jun Lin. (2022) One-Step Integration of Tumor Microenvironment-Responsive Calcium and Copper Peroxides Nanocomposite for Enhanced Chemodynamic/Ion-Interference Therapy. ACS Nano, 16 (1): (617–630). |
| 15. Xionghui Ma, Zejun Jiang, Jianping Li. (2021) An electrochemiluminescence sensor of magnetic glycosyl-imprinted microspheres based on multi-probe signal amplification for CD44v6 determination. SENSORS AND ACTUATORS B-CHEMICAL, 333 (129562). |
| 16. Shuifang Mao, Xiaoli Liu, Wenshui Xia. (2021) Chitosan oligosaccharide-g-linalool polymer as inhibitor of hyaluronidase and collagenase activity. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 166 (1570). |
| 17. Hualu Lai, Xin Ding, Junxian Ye, Jie Deng, Shengmiao Cui. (2021) pH-responsive hyaluronic acid-based nanoparticles for targeted curcumin delivery and enhanced cancer therapy. COLLOIDS AND SURFACES B-BIOINTERFACES, 198 (111455). |
| 18. Chen Di, Shu Yang, Chen Jian, Cao Xianying. (2021) Preparation and in vitro bioactive evaluation of cashew-nut proteins hydrolysate as a potential source of anti-allergy peptides. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 58 (10): (3780-3789). |
| 19. Yunyun Chen, Yanbao Zhao, Xueyan Zou, Lei Sun. (2021) Porous silica nanocarriers with gold/carbon quantum dots for photo-chemotherapy and cellular imaging. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, 61 (102141). |
| 20. Pirah Ayaz, Bingjie Xu, Xiansheng Zhang, Jiping Wang, Dan Yu, Jindan Wu. (2020) A pH and hyaluronidase dual-responsive multilayer-based drug delivery system for resisting bacterial infection. APPLIED SURFACE SCIENCE, 527 (146806). |
| 21. Zhan Wenhua, Li Hanrui, Guo Yingying, Du Getao, Wu Yayan, Zhang Dexin. (2020) Construction of Biocompatible Dual-Drug Loaded Complicated Nanoparticles for in vivo Improvement of Synergistic Chemotherapy in Esophageal Cancer. Frontiers in Oncology, 10 |
| 22. Chen Yunyun, Zhao Yanbao, Sun Lei, Zou Xueyan. (2020) Tri-responsive porous silica carrier with gold nanoparticles for chemophotothermal combination therapy. JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 93 (2): (332-340). |
| 23. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487). |
| 24. Zhu Chen, Shang Deng, De-chao Yuan, Kang Liu, Xiao-cong Xiang, Liang Cheng, Dong-qin Xiao, Li Deng, Gang Feng. (2018) Novel nano-microspheres containing chitosan, hyaluronic acid, and chondroitin sulfate deliver growth and differentiation factor-5 plasmid for osteoarthritis gene therapy. Journal of Zhejiang University-SCIENCE B, 19 (12): ( 910–923). |
| 25. Xiangqian Li, Zhan Zhou, Yiping Tang, Cheng Cheng Zhang, Yuhui Zheng, Jinwei Gao, Qianming Wang. (2018) Modulation of assembly and disassembly of a new tetraphenylethene based nanosensor for highly selective detection of hyaluronidase. SENSORS AND ACTUATORS B-CHEMICAL, 276 (95). |
| 26. Jia Nan Zhang, Bo Zhi Chen, Mohammad Ashfaq, Xiao Peng Zhang, Xin Dong Guo. (2018) Development of a BDDE-crosslinked hyaluronic acid based microneedles patch as a dermal filler for anti-ageing treatment. JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, 65 (363). |
| 27. Li Qiu, Weirui Zhang, Shuyun Wang, Xu Zhang, Yanbao Zhao, Liuqin Cao, Lei Sun. (2017) Construction of multifunctional porous silica nanocarriers for pH/enzyme-responsive drug release. Materials Science & Engineering C-Materials for Biological Applications, 81 (485). |
| 28. He Zhimei, Zhang Penghui, Xiao Yan, Li Jingjing, Yang Fang, Liu Yang, Zhang Jian-Rong, Zhu Jun-Jie. (2018) Acid-degradable gadolinium-based nanoscale coordination polymer: A potential platform for targeted drug delivery and potential magnetic resonance imaging. Nano Research, 11 (2): (929-939). |
| 29. Ning Cui, Junmin Qian, Ting Liu, Na Zhao, Hongjie Wang. (2015) Hyaluronic acid hydrogel scaffolds with a triple degradation behavior for bone tissue engineering. CARBOHYDRATE POLYMERS, 126 (192). |
| 30. Yidan Chen, Tiantian Feng, Xiaohong Zhu, Yuting Tang, Yao Xiao, Xiuhua Zhang, Sheng-Fu Wang, Dong Wang, Wei Wen, Jichao Liang, Huayu Xiong. (2024) Ambient Synthesis of Porphyrin-Based Fe-Covalent Organic Frameworks for Efficient Infected Skin Wound Healing. BIOMACROMOLECULES, 25 (6): (3671-3684). |
| 31. Mengyao Qi, Yi Ke, Yifei Li, Peng Li, Huakuo Zhou, Xia Zhang, Jieshi Chen, Jun Meng. (2025) Au@Pt nanozyme-based smart hydrogel for visual detection of hyaluronic acid. SENSORS AND ACTUATORS B-CHEMICAL, 427 (137144). |
| 32. Tao Meng, Zhengyu Zhao, Mengqiang Song, Rui Wang, Xu Xu, Min Yang, Weihua Li, Shiyong Song. (2025) Carboxybetaine modified chitosan as viscosupplementation for osteoarthritis therapy. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 307 (142155). |
| 33. Yu Pang, Jie Lv, Chengcai He, Chengda Ju, Yulong Lin, Cong Zhang, Meng Li. (2024) Covalent organic frameworks-derived carbon nanospheres based nanoplatform for tumor specific synergistic therapy via oxidative stress amplification and calcium overload. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 661 (908). |
| 34. Siying Huang, Simeng Xiao, Xuehao Li, Ranran Tao, Zhangwei Yang, Ziwei Gao, Junjie Hu, Yan Meng, Guohua Zheng, Xinyan Chen. (2024) Development of Dual-Targeted Mixed Micelles Loaded with Celastrol and Evaluation on Triple-Negative Breast Cancer Therapy. Pharmaceutics, 16 (9): (1174). |
| 35. Shiyi Yao, Hui Yuan, Luqi Yang, Yin Zhang, Hanyu Wang, Renxuan Li, Tingjun Ye, Wenguo Cui, Lei Wang. (2025) Downregulation of the PI3K/AKT/mTOR/MMP-13 pathway for promoting interface healing via lubricating microspheres. Acta Biomaterialia, |
| 36. Jing Zhang, Yunfeng Zeng, Yongyuan Heng, Yu Shen, Ximeng Sun, Yijia Wang, Haiping Zheng, Ming Zeng, Ziyi Yu. (2024) Enzyme-Assisted Activation Technique for Producing Versatile Hydrogel Microparticle Scaffolds with High Surface Chemical Reactivity. ADVANCED FUNCTIONAL MATERIALS, (2400858). |
| 37. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li. (2024) Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound. ACS Nano, 18 (28): (18412-18424). |
| 38. Huiping Yang, Yinning Lin, Qian Mo, Zhaoquan Li, Fan Yang, Xinchun Li. (2024) Monitoring Enzymatic Reaction Kinetics and Activity Assays in Confined Nanospace. ANALYTICAL CHEMISTRY, 96 (30): (12404-12413). |
| 39. Shuo Yu, Lu Zhang, Yanshen Yang, Meijuan Wang, Tingting Liu, Wenwen Ji, Yang Liu, Hao Lv, Yang Zhao, Xi Chen, Tinghua Hu. (2024) Polydopamine-Based Resveratrol-Hyaluronidase Nanomedicine Inhibited Pancreatic Cancer Cell Invasive Phenotype in Hyaluronic Acid Enrichment Tumor Sphere Model. ACS Pharmacology & Translational Science, 7 (4): (1013-1022). |
| 40. Xin Jin, Chengxiong Wei, Kai Li, Peinan Yin, Chengwei Wu, Wei Zhang. (2024) Polyphenol-mediated hyaluronic acid/tannic acid hydrogel with short gelation time and high adhesion strength for accelerating wound healing. CARBOHYDRATE POLYMERS, 342 (122372). |
| 41. Dandan Wei, Guanhua Jiao, Yinghua Tao, Liuxin Yang, Tao Liu, Fengya Jing, Tianzhu Zhang. (2025) Polypropylene Mesh Coated with Dual Cross-Linked Hyaluronic Acid/Polyvinyl Alcohol Composite Hydrogel with Antiadhesion and Angiogenesis Properties for Abdominal Wall Repair. Advanced Materials Technologies, (2401786). |
| 42. Dandan Wei, Yulin Huang, Min Liang, Liuxin Yang, Guanhua Jiao, Yinghua Tao, Li Xu, Tianzhu Zhang, Zhenling Ji. (2024) Polypropylene mesh coated with hyaluronic acid/polyvinyl alcohol composite hydrogel for preventing bowel adhesion. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, (132061). |
| 43. Chao-fan He, Tian-hong Qiao, Xu-chao Ren, Mingjun Xie, Qing Gao, Chao-qi Xie, Peng Wang, Yuan Sun, Huayong Yang, Yong He. (2025) Printability in Multi-material Projection-Based 3-Dimensional Bioprinting. Research, 8 (0613). |
| 44. Zhu Dan, Zhong Hanyan, Shi Jingzhan, Liu Qiang, Wang Yiping. (2025) Ratiometric surface-enhanced Raman scattering quantification of extracellular matrix metalloproteinase-2 activity for tumor diagnosis. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, (1-11). |
| 45. Yingying Chen, Dan Zhu, Hanyan Zhong, Zhixing Gan, Shenfei Zong, Zhuyuan Wang, Yiping Cui, Yiping Wang. (2024) Ultrasensitive Detection of Matrix Metalloproteinase 2 Activity Using a Ratiometric Surface-Enhanced Raman Scattering Nanosensor with a Core–Satellite Structure. ACS Applied Materials & Interfaces, 16 (3): (4160-4168). |
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