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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab220467-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
|
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Ab220467-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$209.90
|
|
|
Ab220467-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$329.90
|
|
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Ab220467-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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mAb (H05/10C6); Mouse anti Human HLA-DR; WB, IF/ICC, ELISA; Unconjugated
| Product Name | HLA-DR Mouse mAb - Primary antibody, specific to Human HLA-DRA |
|---|---|
| Synonyms | DASS-397D15.1 antibody | DR alpha chain antibody | DR alpha chain precursor antibody | DRA_HUMAN antibody | DRB1 antibody | DRB4 antibody | FLJ51114 antibody | Histocompatibility antigen HLA DR alpha antibody | Histocompatibility antigen HLA-DR alpha anti |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell recep |
| Host species | Mouse |
| Specificity | Human HLA-DRA |
| Immunogen | Recombinant protein corresponding to Human HLA-DRA |
| Positive Control | WB: Raji and Ramos whole cell lysates IF/ICC: Raji cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human HLA-DR Antibody, clone H05/10C6, could be used for WB, IF/ICC, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
HLA-DR Mouse mAb (Ab220467) - Western Blot
All lanes: HLA-DR Mouse Antibody (Ab220467) at 1/10000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 30.0 s
HLA-DR Mouse mAb (Ab220467) - IF/ICC analysis of HLA-DR Mouse mAb (Green) in Raji cells. The cells were blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HLA-DR Mouse mAb (Ab220467) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC |
1:500
|
| WB |
1/1000 - 1/20000
|