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HLA-DR Mouse mAb - Primary antibody, specific to Human HLA-DRA

    Application:
  • ELISA
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: H05/10C6
  • Conjugation: Unconjugated
In stock
Item Number
Ab220467
Grouped product items
SKU Size
Availability
Price Qty
Ab220467-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab220467-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$209.90
Ab220467-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90
Ab220467-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (H05/10C6); Mouse anti Human HLA-DR; WB, IF/ICC, ELISA; Unconjugated

Basic Description

Product Name HLA-DR Mouse mAb - Primary antibody, specific to Human HLA-DRA
Synonyms DASS-397D15.1 antibody | DR alpha chain antibody | DR alpha chain precursor antibody | DRA_HUMAN antibody | DRB1 antibody | DRB4 antibody | FLJ51114 antibody | Histocompatibility antigen HLA DR alpha antibody | Histocompatibility antigen HLA-DR alpha anti
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell recep
Host species Mouse
Specificity Human HLA-DRA
Immunogen Recombinant protein corresponding to Human HLA-DRA
Positive Control WB: Raji and Ramos whole cell lysates IF/ICC: Raji cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human HLA-DR Antibody, clone H05/10C6, could be used for WB, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

HLA-DRA Tbio HLA class II histocompatibility antigen, DR alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

HLA-DR Mouse mAb (Ab220467) - Western Blot
All lanes: HLA-DR Mouse Antibody (Ab220467) at 1/10000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 30.0 s 

HLA-DR Mouse mAb (Ab220467) - IF/ICC analysis of HLA-DR Mouse mAb (Green) in Raji cells. The cells were blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HLA-DR Mouse mAb (Ab220467) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1:500

WB

1/1000 - 1/20000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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