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His tag (C-terminal) Mouse mAb (Biotin) - Primary antibody, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.

    Application:
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Species independent
  • Isotype: Mouse IgG1
  • Clone Number: IJ08/4E6
  • Conjugation: Biotin
In stock
Item Number
Ab222055
Grouped product items
SKU Size
Availability
Price Qty
Ab222055-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$83.90
Ab222055-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
Ab222055-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$398.90
Ab222055-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb (IJ08/4E6); Mouse anti His tag (C-terminal) (Biotin); WB, IF/ICC

View related series
Biotin (52) His tag (9)

Basic Description

Product Name His tag (C-terminal) Mouse mAb (Biotin) - Primary antibody, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized met
Host species Mouse
Specificity Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
Immunogen A recombinant protein containing the sequence HHHHHH
Positive Control WB: His-transfected HEK293 whole cell lysates; C-terminal His fusion proteins
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Mouse anti His tag (C-terminal) Antibody, clone IJ08/4E6, could be used for WB and IF/ICC.

Product Properties

Light Chain Type kappa
Purification Method Protein A purified
Concentration 0.5 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Images

His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) - Western Blot
All lanes: His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: SA-HRP (np156148) at 1/10000 dilution

Predicted band size: 113 kDa
Observed band size: 52, 113 kDa
Exposure time: 120.0 s


Application

Application Dilution info
WB

1/1000 - 1/10000

IF/ICC

1/100-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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