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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab222055-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$83.90
|
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Ab222055-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$249.90
|
|
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Ab222055-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$398.90
|
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Ab222055-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
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mAb (IJ08/4E6); Mouse anti His tag (C-terminal) (Biotin); WB, IF/ICC
| Product Name | His tag (C-terminal) Mouse mAb (Biotin) - Primary antibody, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins. |
|---|---|
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized met |
| Host species | Mouse |
| Specificity | Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins. |
| Immunogen | A recombinant protein containing the sequence HHHHHH |
| Positive Control | WB: His-transfected HEK293 whole cell lysates; C-terminal His fusion proteins |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti His tag (C-terminal) Antibody, clone IJ08/4E6, could be used for WB and IF/ICC. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein A purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C. Protect from light. Avoid freeze/thaw cycles. |
His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) - Western Blot
All lanes: His tag (C-terminal) Mouse mAb (Biotin) (Ab222055) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: SA-HRP (np156148) at 1/10000 dilution
Predicted band size: 113 kDa
Observed band size: 52, 113 kDa
Exposure time: 120.0 s
| Application | Dilution info |
|---|---|
| WB |
1/1000 - 1/10000
|
| IF/ICC |
1/100-1/1000
|