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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab176449-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$10.90
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Ab176449-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$39.90
|
|
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Ab176449-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$169.90
|
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pAb; Goat Anti-Rabbit IgG H&L Antibody (Alkaline Phosphatase); WB, ELISA
| Product Name | Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) - Secondary antibody, specific to Rabbit IgG |
|---|---|
| Specifications & Purity | ExactAb™, Validated, See COA |
| Host species | Goat |
| Specificity | Rabbit IgG |
| Immunogen | Rabbit IgG |
| Conjugation | Alkaline Phosphatase |
| Grade | ExactAb™, Validated |
| Product Description |
Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for WB and ELISA. |
| SDS-PAGE | 150kDa |
|---|---|
| Purification Method | Antigen affinity purified |
| Concentration | See COA |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C. Avoid freeze/thaw cycle. Protect from light. |
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - Western Blot
All lanes: beta Actin Antibody (Ab090999) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) at 1/20000 dilution
Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 0.6 s
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - ELISA
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was tested by direct ELISA. The plate was coated with 0.5 µg/mL (100 µL/well) Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was used as the secondary antibody. Detection of alkaline phosphatase (AP) was performed using pNPP (P494193) after 30 minutes incubated at room temperature.
| Application | Dilution info |
|---|---|
| ELISA | 1:1000-1:20000 |
| WB | 1:2000-1:40000 |