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Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) - Secondary antibody, specific to Rabbit IgG

    Application:
  • ELISA
  • WB
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: Alkaline Phosphatase
In stock
Item Number
Ab176449
Grouped product items
SKU Size
Availability
Price Qty
Ab176449-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$10.90
Ab176449-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab176449-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$169.90

pAb; Goat Anti-Rabbit IgG H&L Antibody (Alkaline Phosphatase); WB, ELISA

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) - Secondary antibody, specific to Rabbit IgG
Specifications & Purity ExactAb™, Validated, See COA
Host species Goat
Specificity Rabbit IgG
Immunogen Rabbit IgG
Conjugation Alkaline Phosphatase
Grade ExactAb™, Validated
Product Description

Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for WB and ELISA.

Product Properties

SDS-PAGE 150kDa
Purification Method Antigen affinity purified
Concentration See COA
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - Western Blot
All lanes: beta Actin Antibody (Ab090999) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) at 1/20000 dilution
Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.

Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 0.6 s

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) - ELISA
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was tested by direct ELISA. The plate was coated with 0.5 µg/mL (100 µL/well)  Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (Ab176449) was used as the secondary antibody. Detection of alkaline phosphatase (AP) was performed using pNPP (P494193) after 30 minutes incubated at room temperature.

Application

Application Dilution info
ELISA

1:1000-1:20000

WB

1:2000-1:40000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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