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Goat Anti-Rabbit IgG H&L (AF680) - Secondary antibody, specific to Rabbit IgG

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Application:

IF/ICC

Host species: Goat
Reactive species: Rabbit
Isotype: Goat IgG
Conjugation: AF680

In stock

Item Number

Ab217291

Grouped product items
SKU	Size	Availability	Price
Ab217291-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$39.90
Ab217291-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$99.90
Ab217291-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$399.90

pAb; Goat Anti-Rabbit IgG H&L Antibody (AF680); IF/ICC

View related series

AF680 (4) Goat Anti-Rabbit IgG H&L Antibody (15)

Basic Description

Product Name	Goat Anti-Rabbit IgG H&L (AF680) - Secondary antibody, specific to Rabbit IgG
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Biochemical and Physiological Mechanisms	IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
Host species	Goat
Specificity	Rabbit IgG
Immunogen	Rabbit IgG
Conjugation	AF680
Excitation(nm)	Red Laser(633nm)
Emission(nm)	704nm
Ex/Em(nm)	Ex:681nm;Em:704nm
Grade	ExactAb™, Validated
Product Description	Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for IF/ICC.

Product Properties

SDS-PAGE	150kDa
Purification Method	Antigen affinity purified
Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF/ICC: Goat Anti-Rabbit IgG H&L (AF680) (Ab217291)

Goat Anti-Rabbit IgG H&L (AF680) (Ab217291) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF680) (Ab217291) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

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