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Goat Anti-Mouse IgG H&L (AF660) - Secondary antibody

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Application:

Flow Cytometry
IF/ICC

Host species: Goat
Reactive species: Mouse
Isotype: Goat IgG
Conjugation: 废弃PE. Ex: 488nm, Em: 575nm

In stock

Item Number

Ab217311

Grouped product items
SKU	Size	Availability	Price
Ab217311-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$39.90
Ab217311-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$149.90
Ab217311-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$569.90

pAb; Goat Anti-Mouse IgG H&L Antibody (AF660); IF/ICC, Flow Cytometry

View related series

AF660 (4) Goat Anti-Mouse IgG (1)

Basic Description

Product Name	Goat Anti-Mouse IgG H&L (AF660) - Secondary antibody
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Host species	Goat
Immunogen	Mouse IgG
Conjugation	废弃PE. Ex: 488nm, Em: 575nm
Excitation(nm)	Red Laser(633nm)
Emission(nm)	691nm
Ex/Em(nm)	Ex:663nm;Em:691nm
Grade	ExactAb™, Validated
Product Description	Goat Anti-Mouse IgG H&L Antibody, Secondary antibody, could be used for Flow Cytometry and IF/ICC.

Product Properties

Purification Method	antigen affinity chromatography
Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF/ICC : Goat Anti-Mouse IgG H&L (AF660) (Ab217311)

Goat Anti-Mouse IgG H&L (AF660) (Ab217311) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with 1 μg/mL Catumaxomab (anti-CD3&EpCAM) (Ab175489) in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF660) (Ab217311) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (AF660) (Ab217311)

Goat Anti-Mouse IgG H&L (AF660) (Ab217311) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (AF660) (Ab217311) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	1/100-1/1000
IF/ICC	1/100-1/1000

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