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Goat Anti-Human IgG (APC) (Ready to use) - Secondary antibody, specific to Human IgG

COA

Grade & Purity:

Validated

ExactAb™

See COA

Application:

Flow Cytometry
IF/ICC

Host species: Goat
Reactive species: Human
Isotype: Goat IgG
Conjugation: APC

In stock

Item Number

Ab220092

Grouped product items
SKU	Size	Availability	Price	Qty
Ab220092-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$15.90
Ab220092-10×1ml	10×1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90

pAb; Goat Anti-Human IgG (APC) (Ready to use); Flow Cytometry, IF/ICC

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APC (159) Goat Anti-Human IgG (21) Ready to use (23)

Basic Description

Product Name	Goat Anti-Human IgG (APC) (Ready to use) - Secondary antibody, specific to Human IgG
Specifications & Purity	ExactAb™, Validated, See COA
Host species	Goat
Specificity	Human IgG
Conjugation	APC
Excitation(nm)	Red Laser(633nm)
Emission(nm)	660nm
Ex/Em(nm)	Ex:650nm;Em:660nm
Grade	ExactAb™, Validated
Product Description	Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from human. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.

Product Properties

Purification Method	antigen affinity chromatography
Concentration	See COA
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.

Images

IF/ICC: Goat Anti-Human IgG (APC) (Ready to use) (Ab220092)

Goat Anti-Human IgG (APC) (Ready to use) (Ab220092) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Human IgG (APC) (Ready to use) (Ab220092) at 1mL/well for 1 hour at room temperature in the dark (red). A 24-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG (APC) (Ready to use) (Ab220092)

Goat Anti-Human IgG (APC) (Ready to use) (Ab220092) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Goat Anti-Human IgG (APC) (Ready to use) (Ab220092) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
IF/ICC	Use at an assay dependent volume.
Flow Cytometry	100μL/test

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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