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Exonuclease III - EnzymoPure™, BioReagent, for DNA synthesis, Suitable for molecular biology, PCR Reagent, For In Vitro Transcription, for DNA and RNA applications, 100 U/μl, high purity

COA

Grade & Purity:

for molecular biology

for DNA and RNA applications

100 U/μl

In stock

Item Number

rp216608

Grouped product items
SKU	Size	Availability	Price	Qty
rp216608-5KU	5KU	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
rp216608-25KU	25KU	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$159.90

View related series

Nuclease (7)

Basic Description

Synonyms

Exonuclease III

Specifications & Purity

EnzymoPure™, BioReagent, for DNA synthesis, Suitable for molecular biology, PCR Reagent, For In Vitro Transcription, for DNA and RNA applications, 100 U/μl

Stability And Storage

Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Storage Temp

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for DNA and RNA applications, for DNA synthesis, for molecular biology, PCR Reagent, 废弃Ph. Eur., EnzymoPure™

Product Description

Storage buffer: 50mM Tris, 50mM KCl, 1mM DTT, 0.05mM EDTA, 50% Glycerol, 200 μg/ml HSA, pH 8.0

Exonuclease III has 3 '→5' exonuclease activity on double-stranded DNA, which can degrade flat end, 3 'end dented and incised DNA, and degrade DNA molecules from the 3' end. For 3 'protruding ends, especially protruding DNA molecules 4 nt or longer cannot be cut at all. In addition, the active site of the enzyme depends on the helical structure and varies according to the sequence (C>A=T>G).

Product composition:

rp216608	Component	5KU	25KU	Storage
rp216608A	Exonuclease III	50 μL	250 μL	-20°C. Avoid freeze/thaw cycle
rp216608B	10×Exonuclease III Reaction Buffer	750 μL	3.75 mL	-20°C. Avoid freeze/thaw cycle

Source
E.coli

Enzyme Activity Definition
The amount of enzyme required to catalyze the production of 1 nmol acid-soluble total nucleotide in a 50 μl reaction system at 37℃ for 30 min is defined as 1 unit (U).

Applications

(1) Non-directional nested deletion;
(2) Site-directed mutagenesis;
(3) Preparation of chain specific probes;
(4) Preparation of single-stranded substrates for dideoxy sequencing.
Protocol
(1) Configure the reaction system, as shown in the following table

Component	Volume
DNA	5 μg
10×Exonuclease III Reaction Buffer	5 μl
Exonuclease III	0.5 μl
Nuclease-free Water	Up to 50 μl

(2) Incubation at 37 "C for 30min.
(3) Incubation at 75°C for 10 min terminated the reaction.
Cautions
(1) The reaction temperature, the concentration of salt ions in the system and the ratio of enzyme to DNA will affect the activity of the enzyme.
(2) This product is for scientific research only and shall not be used for other purposes.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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Exonuclease III - EnzymoPure™, BioReagent, for DNA synthesis, Suitable for molecular biology, PCR Reagent, For In Vitro Transcription, for DNA and RNA applications, 100 U/μl, high purity

Basic Description

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews