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Basic Description
| Storage Temp | Store at -20°C,Desiccated |
|---|---|
| Shipped In |
Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available. |
| Product Description |
Product introduction： DyLight 550 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 550 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes. provides vibrant orange-to-red fluorescence with better performance than other rhodamine derivatives including Alexa Fluor™ 555, TRITC and Cy3™ dye for fluorescent applications over a broad pH range (pH 4-9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. Features of DyLight 550 Maleimide: • High performance—DyLight 550 shows brighter fluorescence than Alexa Fluor 555, TRITC and Cy3 dye • Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH) • Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling • Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency Applications: • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies DyLight 550 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more aboutmaleimide chemistry. |
Product Properties
| Reactivity | Thiol |
|---|
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
| Solubility | DMF(Dimethylformamide) |
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Solution Calculators
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