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DM2000

COA

Grade & Purity:

for molecular biology

BioReagent

for electrophoresis

ready-to-use

for NA electrophoresis

100-2000bp, 6 bands, Blue, 100bp/ 250bp/ 500bp/ 750bp/ 1000bp/ 2000bp

In stock

Item Number

D665544

Grouped product items
SKU	Size	Availability	Price	Qty
D665544-100T	100T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$23.90
D665544-500T	500T	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$76.90

View related series

DNA Electrophoresis (9) DNA Ladder (9) DNA Marker (9) DNA molecular weight standard (10) Nucleic acid related (20)

Basic Description

Synonyms

2kb DNA Ladder | 2kb DNA Marker | DNA Marker (100-2000 bp) | D2000 DNA Marker(100-2000bp) | DNA Marker DL2000

Specifications & Purity

BioReagent, ready-to-use, suitable for electrophoresis, Suitable for molecular biology, for NA electrophoresis, 100-2000bp, 6 bands, Blue, 100bp/ 250bp/ 500bp/ 750bp/ 1000bp/ 2000bp

Stability And Storage

Store at room temperature short term (3-6 months). Store at 4℃ long term (24 months). Store at -20℃ long term (60 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Storage Temp

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for electrophoresis, for molecular biology, for NA electrophoresis, ready-to-use

Product Description

Physical appearance: Liquid
Storage buffer: 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6

Product Description:
1. This product consists of 6 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 2000 bp, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, and 2000 bp. The 750 bp band is highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.
2. In 5 ul of this product, the content of the regular bands is approximately 30 ng, while the highlighted band contains about 75 ng.
3. The product is preserved in 1x Loading Buffer and can be directly used for electrophoresis, making it convenient to use.
4. This product is not suitable for polyacrylamide gel electrophoresis.
Recommended Electrophoresis Buffer and Agarose Gel Concentration:
This product is recommended to be used with 1x TAE electrophoresis buffer, with a suggested agarose concentration of 1.5% to 2.0%. For electrophoresis of smaller fragments, it is recommended to use GelRed nucleic acid dye.
Usage Instructions:
1. Prepare an agarose gel containing a nucleic acid dye, such as EB or GelRed.
2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.5% to 2.0%.
3. It is suggested to use 1x TAE buffer, with an electrophoresis voltage not exceeding 10 v/cm.
4. For common 3.5 mm sample wells, a loading volume of 3 to 5 ul is recommended, with an appropriate increase for wider gel wells.
5. Run the electrophoresis to the appropriate distance: For nucleic acid dyes such as EB, Goldview, and GRBlue, the bromophenol blue front should not exceed two-thirds of the gel, otherwise, smaller fragments may weaken due to the detachment of the nucleic acid dye from DNA. For dyes like GelRed, longer distances can be used as long as the smallest fragment does not run out of the gel. Generally, the bromophenol blue indicator band should be at least 1 cm away from the edge of the gel.
6. After electrophoresis, observe the bands under a UV lamp.
7. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, containing both bromophenol blue and xylene cyanol as indicators.

Product component

D665544	Component	100 T	500T	Storage
D665544A	DNA Ladder (100-2000bp)	500 μL	5× 500 μL	-20℃. Avoid freeze/thaw cycle.
D665544B	5xLoading buffer	1mL	5×1 mL	-20℃. Avoid freeze/thaw cycle.

Images

1.5% Agarose in TAE: DNA Ladder (100-2000bp) (D665544)

DNA Ladder (100-2000bp) (D665544) - 1.5% Agarose in TAE
Samples: Lysates at 5 µL per lane
DNA Ladder (100-2000bp) (D665544) consists of 6 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 2000 bp, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, and 2000 bp. The 750 bp band is highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

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*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

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Reconstitution Calculator

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DM2000

Basic Description

Images

Names and Identifiers

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews