Grouped product items

SKU

Size

Availability

Price

Qty

D420461-1ml

1ml

Available within 8-12 weeks(?)

$240.90

Basic Description

Synonyms	Dihydroethidium \| 104821-25-2 \| Hydroethidine \| 5-ethyl-6-phenyl-5,6-dihydrophenanthridine-3,8-diamine \| PD-MY 003 \| 5-ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine \| 5-ethyl-6-phenyl-6H-phenanthridine-3,8-diamine \| 3pm1 \| Dihydroethidium, >=95% \| Hydroethidine;PD-M
Specifications & Purity	10mM in DMSO
Storage Temp	Protected from light,Store at -80°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	Hydroethidine operates effectively as a probe for measurement of reactive oxygen species. The dye enters cells freely and is dehydrogenated to ethidium bromide. The probe has been used extensively with NK cell and as a vital dye for identification of proliferation and hypoxic cells in tumors. Studies have been performed using neutrophils and endothelial cells as well as HL60 cells and macrophages. A major advantage of this probe is its ability to distinguish between superoxide and H2O2. Fluorescence emission occurs at around 600 nm. Redox indicator. Blue fluorescence until oxidized to ethidium

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organoheterocyclic compounds
  - Class Quinolines and derivatives
    - Subclass Phenylquinolines

Kingdom	Organic compounds
Superclass	Organoheterocyclic compounds
Class	Quinolines and derivatives
Subclass	Phenylquinolines
Intermediate Tree Nodes	Not available
Direct Parent	Phenylquinolines
Alternative Parents	Phenanthridines and derivatives Aminoquinolines and derivatives Dialkylarylamines Aralkylamines Benzene and substituted derivatives Azacyclic compounds Primary amines Organopnictogen compounds Hydrocarbon derivatives
Molecular Framework	Aromatic heteropolycyclic compounds
Substituents	Phenylquinoline - Benzoquinoline - Phenanthridine - Aminoquinoline - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Aralkylamine - Benzenoid - Monocyclic benzene moiety - Tertiary amine - Azacycle - Amine - Primary amine - Hydrocarbon derivative - Organonitrogen compound - Organopnictogen compound - Organic nitrogen compound - Aromatic heteropolycyclic compound
Description	This compound belongs to the class of organic compounds known as phenylquinolines. These are heterocyclic compounds containing a quinoline moiety substituted with a phenyl group.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Names and Identifiers

IUPAC Name	5-ethyl-6-phenyl-6H-phenanthridine-3,8-diamine
INCHI	InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChIKey	XYJODUBPWNZLML-UHFFFAOYSA-N
Smiles	CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4
Isomeric SMILES	CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4
WGK Germany	3
Molecular Weight	315.41
Beilstein	5107482
Reaxy-Rn	5107482
Reaxys-RN_link_address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=5107482&ln=

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Chemical and Physical Properties

Sensitivity	Air sensitive.Light sensitive.
Molecular Weight	315.400 g/mol
XLogP3	3.900
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Exact Mass	315.174 Da
Monoisotopic Mass	315.174 Da
Topological Polar Surface Area	55.300 Å²
Heavy Atom Count	24
Formal Charge	0
Complexity	419.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	1
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	1

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Tongsheng Huang, Xin Hu, Shiyu Huang, Dong Liu, Lihua Chen, Qinghua Wu, Weifeng Zhu, Xiaoyong Zhang, Meiying Liu, Yen Wei. (2024) One-step synthesis of cyclodextrin-based fluorescent hyperbranched polymer as type I photosensitizer for effective photodynamic therapy. DYES AND PIGMENTS, 223 (111928).
2. Lei Qian, Hu Xu, Ruqiang Yuan, Weijing Yun, Yufang Ma. (2024) Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction. BIOMEDICINE & PHARMACOTHERAPY, 170 (116000).
3. Ping Ping, Shasha Guan, Chaoxue Ning, Ting Yang, Yali Zhao, Pei Zhang, Zhitao Gao, Shihui Fu. (2023) Fabrication of blended nanofibrous cardiac patch transplanted with TGF-β3 and human umbilical cord MSCs-derived exosomes for potential cardiac regeneration after acute myocardial infarction. Nanomedicine-Nanotechnology Biology and Medicine, 54 (102708).
4. Mengmeng Pan, Ming Wang, Linjiao Yang, Yongli Song, Ming Jiang, Xu Yu, Li Xu. (2023) Construction of Metal Organic Framework-Derived Fe-N-C Oxidase Nanozyme for Rapid and Sensitive Detection of Alkaline Phosphatase. Nanomaterials, 13 (18): (2496).
5. Ke Xue, Yongfei Zhao, Saidong Sun, Yuanhang Li, Jiankang Liang, Zhengjian Qi. (2023) NIR-AIEgens nanospheres featuring high-fidelity dynamic lipid droplet targeting, expediting ferroptosis to annihilating tumor in hypoxia. CHEMICAL ENGINEERING JOURNAL, 470 (144125).
6. Zhen Li, Yuanji Feng, Sijia Zhang, Tong Li, Huixin Li, Dianwei Wang, Kai Hao, Chaoliang He, Huayu Tian, Xuesi Chen. (2023) A Multifunctional Nanoparticle Mitigating Cytokine Storm by Scavenging Multiple Inflammatory Mediators of Sepsis. ACS Nano, 17 (9): (8551–8563).
7. Yi Zhao, Yang Liu, Yuying Wang, Shulan Li, Yi Liu, Zhong Lin Wang, Peng Jiang. (2023) The process of free radical generation in contact electrification at solid-liquid interface. Nano Energy, 112 (108464).
8. Yizhong Shen, Xiang Gao, Huanhuan Chen, Yunlong Wei, Hui Yang, Ying Gu. (2023) Ultrathin C3N4 nanosheets-based oxidase-like 2D fluorescence nanozyme for dual-mode detection of organophosphorus pesticides. JOURNAL OF HAZARDOUS MATERIALS, 451 (131171).
9. Xiaoyi Xie, Xiaofang Chen, Yaohui Wang, Maosen Zhang, Yuxiu Fan, Xiupei Yang. (2023) High-loading Cu single-atom nanozymes supported by carbon nitride with peroxidase-like activity for the colorimetric detection of tannic acid. TALANTA, 257 (124387).
10. Na An, Yan Gao, Ying Shi, Xingxing Du, Jianing Cheng, Shuanglong Tang, Pengfei Liu, Huike Yang, Chongshen Guo. (2023) Nickel phosphide for MRI-guided synergistic photo-immunotherapy. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY, 151 (150).
11. Ji Ma, Yang Deng, Tingting Yang, Maoru Li, Jing Shang. (2023) Esculetin Alleviates Nonalcoholic Fatty Liver Disease on High-Cholesterol-Diet-Induced Larval Zebrafish and FFA-Induced BRL-3A Hepatocyte. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2): (1593).
12. Qingqing Liu, Chenchen Xu, Su Chu, Shuai Li, Fengxiang Wang, Yanmei Si, Guojiang Mao, Choufei Wu, Hua Wang. (2022) Covalent organic framework-loaded silver nanoparticles as robust mimetic oxidase for highly sensitive and selective colorimetric detection of mercury in blood. Journal of Materials Chemistry B, 10 (48): (10075-10082).
13. Pinghua Ling, Pei Yang, Xianping Gao, Xinyu Sun, Feng Gao. (2022) ROS generation strategy based on biomimetic nanosheets by self-assembly of nanozymes. Journal of Materials Chemistry B, 10 (46): (9607-9612).
14. Yecan Pan, Rui Weng, Linghao Zhang, Jing Qiu, Xinlu Wang, Guangqin Liao, Zhaohui Qin, Lingpu Zhang, Haihua Xiao, Yongzhong Qian, Xin Su. (2022) Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response. Nano Today, 46 (101573).
15. Chenghu Dai, Hao Jia, Wubin Wu, Baipeng Yin, Hong Wang, Ling Wang, Yeteng Zhong, Zihua Wang, Chuang Zhang, Jiannian Yao. (2022) Optically Triggering and Monitoring Single-Cell-Level Metabolism Using Ormosil-Decorated Ultrathin Fibers. LANGMUIR, 38 (32): (9844–9852).
16. Hengrui Zhang, Wenya Jiang, Yaou Peng, Jie Yang, Xiaoying Chu, Ziyue Long, Renlong Li, Qiuwei Liang, Hao Suo, Shuting Wang, Mei Yang, Ji Qi, Dan Ding, Ying-Wei Yang, Bailiang Wang. (2022) Killing three birds with one stone: Near-infrared light triggered nitric oxide release for enhanced photodynamic and anti-inflammatory therapy in refractory keratitis. BIOMATERIALS, 286 (121577).
17. Wei Lu, Shuhan Chen, Haifeng Zhang, Jiajun Qiu, Xuanyong Liu. (2022) FeNC single atom nanozymes with dual enzyme-mimicking activities for colorimetric detection of hydrogen peroxide and glutathione. Journal of Materiomics, 8 (1251).
18. Xiaoyu Huang, Wei Zhang, Yaowei Peng, Lu Gao, Fu Wang, Lan Wang, Xunbin Wei. (2022) A Multifunctional Layered Nickel Silicate Nanogenerator of Synchronous Oxygen Self-supply and Superoxide Radical Generation for Hypoxic Tumor Therapy. ACS Nano, 16 (1): (974–983).
19. Huang Rui, Zhao Zizuo, Jiang Xujie, Li Weiwei, Zhang Lidan, Wang Bin, Tie Hongtao. (2021) Liposomal chrysin attenuates hepatic ischaemia-reperfusion injury: possible mechanism via inhibiting NLRP3 inflammasome. JOURNAL OF PHARMACY AND PHARMACOLOGY, 74 (2): (216-226).
20. Jian Li, Tingting Wang, Feng Jiang, Zhangyong Hong, Xinhui Su, Shuang Li, Shoufa Han. (2021) A fluorescence-activatable tumor-reporting probe for precise photodynamic therapy. Journal of Materials Chemistry B, 9 (29): (5829-5836).
21. Yang Deng, Ji Ma, Xin Weng, Yuqin Wang, Maoru Li, Tingting Yang, Zhiyang Dou, Zhiqi Yin, Jing Shang. (2021) Kaempferol-3-O-Glucuronide Ameliorates Non-Alcoholic Steatohepatitis in High-Cholesterol-Diet-Induced Larval Zebrafish and HepG2 Cell Models via Regulating Oxidation Stress. Life-Basel, 11 (5): (445).
22. Jian Li, Tingting Wang, Feng Jiang, Zhangyong Hong, Xinhui Su, Shuang Li, Shoufa Han. (2021) Activatable Dual ROS-Producing Probe for Dual Organelle-Engaged Photodynamic Therapy. ACS Applied Bio Materials, 4 (5): (4618–4628).
23. Xueliang Liu, Yingmin Zhou, Jin Liu, Hongjun Xia. (2021) The intrinsic enzyme mimetic activity of platinum oxide for biosensing of glucose. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 248 (119280).
24. Xu Weiqing, Kang Yikun, Jiao Lei, Wu Yu, Yan Hongye, Li Jinli, Gu Wenling, Song Weiyu, Zhu Chengzhou. (2020) Tuning Atomically Dispersed Fe Sites in Metal–Organic Frameworks Boosts Peroxidase-Like Activity for Sensitive Biosensing. Nano-Micro Letters, 12 (1): (1-12).
25. Shiyan Fu, Ruihao Yang, Lei Zhang, Weiwei Liu, Guangyuan Du, Yang Cao, Zhigang Xu, Hongjuan Cui, Yuejun Kang, Peng Xue. (2020) Biomimetic CoO@AuPt nanozyme responsive to multiple tumor microenvironmental clues for augmenting chemodynamic therapy. BIOMATERIALS, 257 (120279).
26. Yufeng Liu, Yuan Cheng, He Zhang, Min Zhou, Yijun Yu, Shichao Lin, Bo Jiang, Xiaozhi Zhao, Leiying Miao, Chuan-Wan Wei, Quanyi Liu, Ying-Wu Lin, Yan Du, Christopher J. Butch, Hui Wei. (2020) Integrated cascade nanozyme catalyzes in vivo ROS scavenging for anti-inflammatory therapy. Science Advances, 6 (29):
27. Zhangping Lou, Sheng Zhao, Quan Wang, Hui Wei. (2019) N-Doped Carbon As Peroxidase-Like Nanozymes for Total Antioxidant Capacity Assay. ANALYTICAL CHEMISTRY, 91 (23): (15267–15274).
28. Hongwei Song, Zhibo Li, Yinxian Peng, Xin Li, Xuechao Xu, Jianming Pan, Xiangheng Niu. (2019) Enzyme-triggered in situ formation of Ag nanoparticles with oxidase-mimicking activity for amplified detection of alkaline phosphatase activity. ANALYST, 144 (7): (2416-2422).
29. Yan Gao, Fei Wang, Weicheng Huang, Chunyu Yang, Wei Guo, Chuanqi Song, Qun Zhang, Bin Yang, Yanling Xu, Chongshen Guo. (2019) SnxWO3 as a theranostic platform for realizing multi-imaging-guided photothermal/photodynamic combination therapy. Nanoscale, 11 (7): (3300-3310).
30. Yuanlin Huang, Tianran Lin, Li Hou, Fanggui Ye, Shulin Zhao. (2019) Colorimetric detection of thioglycolic acid based on the enhanced Fe3+ ions Fenton reaction. MICROCHEMICAL JOURNAL, 144 (190).
31. Chen Shao, Kun Shang, Huaibao Xu, Yu Zhang, Zhichao Pei, Yuxin Pei. (2018) Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy. International Journal of Nanomedicine, 13 ( 4319–4331).
32. Ze-Yuan Wang, Yi Li, Wen-Qi Chang, Jia-Yi Zheng, Ping Li, Li-Fang Liu, Gui-Zhong Xin. (2018) Development and validation of a LC/MS-based method for the measurement of intracellular superoxide anion. ANALYTICA CHIMICA ACTA, 999 (107).
33. Haiguan Yang, Junqi Zha, Peng Zhang, Yuemei Qin, Tao Chen, Fanggui Ye. (2017) Fabrication of CeVO4 as nanozyme for facile colorimetric discrimination of hydroquinone from resorcinol and catechol. SENSORS AND ACTUATORS B-CHEMICAL, 247 (469).
34. Qiao Chen, Xu Yang, Yao Yu, Xinmei Duan, Rongrong Ni, Guojing Song, Li Zhu, Yuan Zhong, Kai Qu, Xian Qin, Kun Zhang, Yang Luo, Wei Wu. (2025) Biomimetic Cerium-Assisted Supra-Carbon Dots Assembly for Reactive Oxygen Species-Activated Atherosclerosis Theranostic. Small, (2408980).
35. Yanhui Wang, Huayu Fu, Bangqi Huang, Ziyang Liang, Yijun Shen, Yulan Wang, Jun Wu, Yi Zhao. (2024) Hollow mesoporous Prussian blue nanoenzymes as Rapamycin carrier for targeted treatment of ischemia/reperfusion-induced acute kidney injury through pro-mitophagy and oxidative stress alleviation. CHEMICAL ENGINEERING JOURNAL, 497 (155684).
36. Meiying Liu, Tongsheng Huang, Yiping Liu, Lihua Chen, Shijie Liao, Xinxin Gong, Mubarak G. Bello, Weifeng Zhu, Shaorong Huang, Xiaoyong Zhang. (2025) Loading curcumin on hyperbranched polymers functionalized Zein via the phenol-yne click reaction as pH-responsive drug delivery system for chemotherapy and photodynamic therapy. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, (139750).
37. Ming-Li Sun, Jun-Min Dong, Chen Liu, Pu Li, Chao Zhang, Jie Zhen, Wei Chen. (2024) Metformin-mediated protection against doxorubicin-induced cardiotoxicity. BIOMEDICINE & PHARMACOTHERAPY, 180 (117535).
38. Mingdi Yang, Kaiwen Li, Liangchen Zhong, Yingcui Bu, Yingyong Ni, Ting Wang, Jing Huang, Jingyan Zhang, Hongping Zhou. (2024) Molecular engineering to elevate reactive oxygen species generation for synergetic damage on lipid droplets and mitochondria. ANALYTICA CHIMICA ACTA, 1311 (342734).
39. Xianjiao Tang, Liping Chen, Yuhan Wang, Pengwei Chen, Lin-Song Li, Xiaojing Yang, Mei-Xia Zhao. (2024) Multimodal phototherapy agents target lipid droplets for near-infrared imaging-guided type I photodynamic/photothermal therapy. Acta Biomaterialia,
40. Ting Xian, Yilin Liu, Qingtao Song, Jing Li, Wenjuan Liu, Zhongwei Gu. (2024) NIR-Mediated Cu2O/Au Nanomotors for Synergistically Treating Hepatoma Carcinoma Cells. Chemistry-An Asian Journal, (e202301137).

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Dihydroethidium - 10mM in DMSO, high purity , CAS No.104821-25-2