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DAF-2 (DMSO solution) - 5 mM in DMSO, high purity , CAS No.205391-01-1

    Grade & Purity:
  • 5 mM in DMSO
In stock
Item Number
D276597
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D276597-100μg
100μg
Available within 8-12 weeks(?)
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$186.90

Fluorescent nitric oxide probe

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Compound libraries (12325)

Basic Description

Synonyms 4,5-Diaminofluorescein | 2-(3,6-Dihydroxy-9H-xanthen-9-yl)-4,5-diamino-benzoic acid | DTXSID60443252 | AS-78359 | 5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one | DAF-2-CAS 205391-01-1-Calbiochem | Spiro[isobenzofuran-1(3H),9'-[9H]
Specifications & Purity 5 mM in DMSO
Biochemical and Physiological Mechanisms Fluorescent nitric oxide probe. DAF-2 solution. Reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein DAF-2T . Determines nitric oxide synthase activity. Membrance impermeable DAF-2 DA ( ab14;Fluorescent indicator fo
Storage Temp Protected from light,Desiccated,Store at -80°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Note Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.


description:

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T). Can detect NO under neutral conditions (detection limit = 5 nM).

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
Superclass Organoheterocyclic compounds
Class Benzopyrans
Subclass 1-benzopyrans
Intermediate Tree Nodes Dibenzopyrans
Direct Parent Xanthenes
Alternative Parents Diarylethers  Phthalides  Benzofuranones  Isobenzofurans  1-hydroxy-2-unsubstituted benzenoids  Lactones  Carboxylic acid esters  Amino acids and derivatives  Oxacyclic compounds  Primary amines  Organic oxides  Hydrocarbon derivatives  
Molecular Framework Aromatic heteropolycyclic compounds
Substituents Xanthene - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isocoumaran - Isobenzofuran - Phenol - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Carboxylic acid ester - Lactone - Amino acid or derivatives - Ether - Carboxylic acid derivative - Oxacycle - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Organic oxide - Organic nitrogen compound - Organic oxygen compound - Amine - Primary amine - Aromatic heteropolycyclic compound
Description This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors Not available

Names and Identifiers

IUPAC Name 5,6-diamino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
INCHI InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
InChIKey LTYUPYUWXRTNFQ-UHFFFAOYSA-N
Smiles C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O
Isomeric SMILES C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O
Molecular Weight 362.34
Reaxy-Rn 8014486
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=8014486&ln=

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

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Lot Number Certificate Type Date Item
I2207216 Certificate of Analysis Jun 15, 2023 D276597

Chemical and Physical Properties

Solubility Supplied in DMSO (5 mM)
Sensitivity air and light sensitive
Molecular Weight 362.300 g/mol
XLogP3 2.100
Hydrogen Bond Donor Count 4
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 0
Exact Mass 362.09 Da
Monoisotopic Mass 362.09 Da
Topological Polar Surface Area 128.000 Ų
Heavy Atom Count 27
Formal Charge 0
Complexity 589.000
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
The total count of all stereochemical bonds 0
Covalently-Bonded Unit Count 1

Citations of This Product

1. Shuang-Shuang Wan, Jin-Yue Zeng, Han Cheng, Xian-Zheng Zhang.  (2018)  ROS-induced NO generation for gas therapy and sensitizing photodynamic therapy of tumor.  BIOMATERIALS,  185  (51). 

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