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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab183902-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$39.90
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Ab183902-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
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Ab183902-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$119.90
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Ab183902-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$439.90
|
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mAb (EF01/4D12); Mouse anti Human Cyclophilin A; WB, Flow Cytometry, ELISA; Unconjugated
| Product Name | Cyclophilin A Mouse mAb - Primary antibody, specific to Human Cyclophilin A |
|---|---|
| Synonyms | Cyclophilin A antibody | Cyclophilin antibody | CyclophilinA antibody | Cyclosporin A binding protein antibody | Cyclosporin A-binding protein antibody | CYPA antibody | CYPH antibody | Epididymis secretory sperm binding protein Li 69p antibody | HEL S 69 |
| Specifications & Purity | ExactAb™, Validated, Animal Free, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides; Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates i |
| Host species | Mouse |
| Specificity | Human Cyclophilin A |
| Immunogen | Recombinant protein corresponding to Human Cyclophilin A |
| Positive Control | WB: HeLa, K562, HepG2, A549, Jurkat, A431and NIH/3T3 whole cell lysates; Mouse brain and Rat brain tissue Flow Cytometry: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Cyclophilin A Antibody, clone EF01/4D12, could be used for WB, Flow Cytometry, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Cyclophilin A Mouse mAb (Ab183902) - Western Blot
All lanes: Cyclophilin A Mouse Antibody (Ab183902) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 60.0 s
Cyclophilin A Mouse mAb (Ab183902) - Western Blot
All lanes: Cyclophilin A Mouse Antibody (Ab183902) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 90.0 s
Cyclophilin A Mouse mAb (Ab183902) - Western Blot
All lanes: Cyclophilin A Mouse Antibody (Ab183902) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 90.0 s
Cyclophilin A Mouse mAb (Ab183902) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Cyclophilin A Mouse mAb (Ab183902) (Red) at 5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).
| Application | Dilution info |
|---|---|
| WB |
1/1000 - 1/20000
|
| Flow Cytometry | 1/200 - 1/3000 |
Starting at $69.90
Starting at $79.90