Basic Description

Product Name	Collagenase NB 7D From Clostridium Histolyticum - Gentle Grade,≥ 0.2 U/mg, high purity , CAS No.9001-12-1
Synonyms	Collagenase I \| Collagenase Type II \| (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
Grade	sterile, EnzymoPure™
Product Description	Collagenases from Clostridium histolyticum are proteolytic enzymes that cleave peptide bonds in the triple helical collagen molecule of human or animal tissue in situ. They are also referred as crude collagenases, because they consist of collagenase class I and class II, neutral proteases and clostripain in different ratios. The ratio of enzymatic activities can be altered enabling cell isolation from various tissue types. Collagenase NB 7D Gentle Grade is a partly purified collagenase that has slightly higher collagenase activity while containing lower tryptic activities. The product is intended for research use only. Specification Collagenase activity ≥ 0.2 U/mg (PZ acc. to Wünsch) Trypsin-like activities ≤ 0.2 U/mg (BAEE) Ratio Collagenase : Trypsin ≥ 3.0 Application Collagenase NB 7D Gentle Grade is suitable for dissociation of a broad variety of tissue types especially in cases where sensitive conditions are beneficial. Storage conditions Collagenase NB 7D Gentle Grade is provided as a lyophilized conditionspowder. It should be stored at +2 to +8 ℃ in a dry environment. Under these conditions the product is stable until the minimum shelf life stated on the certificate of analysis if repeated opening and closing of the vial is avoided. Instructions for use: General Collagenase NB 7D Gentle Grade is especially designed for isolation of sensitive cells (e.g. dendritic cells, diverse tumor cells) from human or animal tissues. The product exhibits slightly higher collagenase activity and low tryptic activities preserving cell-membrane receptors and other cell-surface proteins. Tissue dissociation Recommended starting concentrations for selected applications: Skin (mouse): 0.18 - 0.22 PZ U/ml Tumor cells (mouse): 0.50 - 0.60 PZ U/ml In general, the appropriate collagenase concentration depends on tissue type and origin as well as on the isolation procedure. Collagenase activity is at an optimum at 37 ℃ and pH 7.4. Working solution To prepare a working solution, the stock solution is diluted with buffer to achieve the required collagenase concentration. The working solu-tion must be constantly stored on ice until use. Inactivation and inhibitors The dissociation process can be reduced, e.g. by cooling down or dilution of the enzyme solution. Collagenase is reversibly inactivated at high pH values and irrevers-ibly inactivated at low pH values. Inhibitors of collagenase include cysteine or chelating agents like EDTA. Important note Collagenase NB 7D Gentle Grade is intended for research use only.
Specifications & Purity	EnzymoPure™, sterile, ≥0.2 U/mg powder
Biochemical and Physiological Mechanisms	Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly
Bioactivity	≥0.2 U/mg powder
Accession #	P43153,Q46085,Q9X721
Predicted molecular weight	70–120 kDa

Associated Targets(Human)

HT-29 (80576 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 Activities)

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SK-MEL-5 (47095 Activities)

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A549 (127892 Activities)

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Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Storage and Shipping

Concentration	≥0.2 U/mg powder
Reconstitution	It is recommended to prepare a stock solution by dissolving collagenase NB in an appropriate volume of buffer (e.g., HBSS, PBS, Tris, or Ringer’s solution). The prepared enzyme should be sterile-filtered (if not sterile), aliquoted and stored at -20°C. Our stability studies indicate that the enzyme remains stable for up to one year under these conditions, provided that repeated freeze-thaw cycles are avoided.
Storage Temp	Store at 2-8°C
Shipped In	Wet ice
Stability And Storage	Store at 2-8℃ long term (1 year). Upon reconstitution, it is recommended to aliquot.
CAS	9001-12-1
Enzyme Commission Number	EC 3.4.24.3
Unit definition	The hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25℃, pH 7.1.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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Analytical Chart:

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, 165 (115096).
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 242 (124820).
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 668 (131410).
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2023) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 631 (122489).
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2023) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, 18 (100506).
6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002).
7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 31 (17): (2199-2219).
8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2020) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, 109 (110538).
9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 134 (838).
10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487).
11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, 92 (447).
12. Liuxin Yang, Fengya Jing, Dandan Wei, Xiaocong Zhao, Yinghua Tao, Tao Liu, Tianzhu Zhang. (2025) Assembled granular hydrogels loaded with growth factors for enhanced mesenchymal stem cell therapy in abdominal wall defect repair. JOURNAL OF CONTROLLED RELEASE, 381 (113630).
13. Tao Li, Yongtao Yan, Linlin Han, Mingkun Li, Shiyu Liu, XinYang Zhou, Jungjoon Lee, Xiaomin Li, Yuqing Zhao. (2024) Ginseng fruit rare saponins (GFRS), a promising anti-wrinkle agent: Evidence of its antioxidant effect and its capacity to prevent matrix metalloproteinase (MMPs) expression in vitro and in vivo. INDUSTRIAL CROPS AND PRODUCTS, 215 (118716).
14. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li. (2024) Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound. ACS Nano, 18 (28): (18412-18424).
15. Chen Zhengfang, He Mudan, Wang Houpeng, Li Xuehui, Qin Ruirui, Ye Ding, Zhai Xue, Zhu Junwen, Zhang Quanqing, Hu Peng, Shui Guanghou, Sun Yonghua. (2024) Intestinal DHA-PA-PG axis promotes digestive organ expansion by mediating usage of maternally deposited yolk lipids. Nature Communications, 15 (1): (1-15).
16. Liuxin Yang, Dandan Wei, Yinghua Tao, Fengya Jing, Tao Liu, Guanhua Jiao, Tianzhu Zhang. (2024) Spatiotemporal delivery of microenvironment responsive hydrogel incorporated with stem cells-loaded porous microspheres for abdominal wall repair. CHEMICAL ENGINEERING JOURNAL, 497 (154435).
17. Liuxin Yang, Guanhua Jiao, Dandan Wei, Tianzhu Zhang. (2025) Synthesis and characterization of photocrosslinked gelatin-silk fibroin composite microspheres for mesenchymal stem cell expansion and osteogenic differentiation. Materials Today Communications, 44 (111912).

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SKU	Size	Availability	Price
C278996-10mg	10mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
C278996-100mg	100mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$209.90
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C278996-1g	1g	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,229.90

Collagenase NB 7D From Clostridium Histolyticum - Gentle Grade,≥ 0.2 U/mg, high purity , CAS No.9001-12-1

Basic Description

Associated Targets(Human)

Mechanisms of Action

Storage and Shipping

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Citations of This Product

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews