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Basic Description
| Product Name | Collagenase from Clostridium histolyticum(Type 1) - ≥125 units/mg dry weight, high purity , CAS No.9001-12-1 |
|---|---|
| Synonyms | Collagenase I |
| Grade | EnzymoPure™ |
| Product Description |
Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4. Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities. |
| Specifications & Purity | EnzymoPure™, ≥125 units/mg dry weight |
| Accession # | P04180 |
Associated Targets(Human)
HT-29 (80576 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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MCF7 (126967 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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SK-MEL-5 (47095 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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A549 (127892 Activities)
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Mechanisms of Action
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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Storage and Shipping
| Concentration | ≥125 units/mg dry weight |
|---|---|
| Storage Temp | Store at 2-8°C |
| Shipped In | Wet ice |
| CAS | 9001-12-1 |
| Enzyme Commission Number | EC: 3.4.24.3 |
| Unit definition | One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5. |
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 B2510182 |
Certificate of Analysis | Dec 27, 2024 | C128702 |
| B2510183 |
Certificate of Analysis | Dec 27, 2024 | C128702 |
| J2428505 |
Certificate of Analysis | Oct 18, 2024 | C128702 |
| J2428550 |
Certificate of Analysis | Oct 18, 2024 | C128702 |
| F2405323 |
Certificate of Analysis | May 20, 2024 | C128702 |
| F2405324 |
Certificate of Analysis | May 20, 2024 | C128702 |
| F2405325 |
Certificate of Analysis | May 20, 2024 | C128702 |
| B2301597 |
Certificate of Analysis | Feb 07, 2023 | C128702 |
| F2301047 |
Certificate of Analysis | Jul 01, 2022 | C128702 |
| I2321024 |
Certificate of Analysis | Jul 01, 2022 | C128702 |
| J2323083 |
Certificate of Analysis | Jul 01, 2022 | C128702 |
| G2222237 |
Certificate of Analysis | Jul 01, 2022 | C128702 |
| G2222239 |
Certificate of Analysis | Jul 01, 2022 | C128702 |
| G2222208 |
Certificate of Analysis | Jul 01, 2022 | C128702 |
| A2403040 |
Certificate of Analysis | Jul 01, 2022 | C128702 |
| D2127056 |
Certificate of Analysis | May 05, 2021 | C128702 |
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Collagenase from Clostridium histolyticum(Type 4)Starting at $412.90
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Collagenase from Clostridium histolyticum(Animal Origin Free,C)
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Collagenase from Clostridium histolyticum(Type 2)
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Collagenase from Clostridium histolyticum(Animal Origin Free,A)
Starting at $368.90
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Collagenase from Clostridium histolyticum(Animal Origin Free,B)
Starting at $368.90
Citations of This Product
| 1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, 165 (115096). |
| 2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 242 (124820). |
| 3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 668 (131410). |
| 4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2023) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 631 (122489). |
| 5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2023) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, 18 (100506). |
| 6. Congying Wei, Weili Shi, Chuangqi Zhao, Shuai Yang, Jiajia Zheng, JinPan Zhong, Tianyi Zhao, Simin Kong, Xi Gong, Mingjie Liu. (2022) Superwetting Injectable Hydrogel with Ultrastrong and Fast Tissue Adhesion for Minimally Invasive Hemostasis. Advanced Healthcare Materials, 12 (3): (2201799). |
| 7. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002). |
| 8. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 31 (17): (2199-2219). |
| 9. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2020) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, 109 (110538). |
| 10. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 134 (838). |
| 11. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487). |
| 12. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, 92 (447). |
| 13. Liuxin Yang, Fengya Jing, Dandan Wei, Xiaocong Zhao, Yinghua Tao, Tao Liu, Tianzhu Zhang. (2025) Assembled granular hydrogels loaded with growth factors for enhanced mesenchymal stem cell therapy in abdominal wall defect repair. JOURNAL OF CONTROLLED RELEASE, 381 (113630). |
| 14. Tao Li, Yongtao Yan, Linlin Han, Mingkun Li, Shiyu Liu, XinYang Zhou, Jungjoon Lee, Xiaomin Li, Yuqing Zhao. (2024) Ginseng fruit rare saponins (GFRS), a promising anti-wrinkle agent: Evidence of its antioxidant effect and its capacity to prevent matrix metalloproteinase (MMPs) expression in vitro and in vivo. INDUSTRIAL CROPS AND PRODUCTS, 215 (118716). |
| 15. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li. (2024) Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound. ACS Nano, 18 (28): (18412-18424). |
| 16. Chen Zhengfang, He Mudan, Wang Houpeng, Li Xuehui, Qin Ruirui, Ye Ding, Zhai Xue, Zhu Junwen, Zhang Quanqing, Hu Peng, Shui Guanghou, Sun Yonghua. (2024) Intestinal DHA-PA-PG axis promotes digestive organ expansion by mediating usage of maternally deposited yolk lipids. Nature Communications, 15 (1): (1-15). |
| 17. Liuxin Yang, Dandan Wei, Yinghua Tao, Fengya Jing, Tao Liu, Guanhua Jiao, Tianzhu Zhang. (2024) Spatiotemporal delivery of microenvironment responsive hydrogel incorporated with stem cells-loaded porous microspheres for abdominal wall repair. CHEMICAL ENGINEERING JOURNAL, 497 (154435). |
| 18. Liuxin Yang, Guanhua Jiao, Dandan Wei, Tianzhu Zhang. (2025) Synthesis and characterization of photocrosslinked gelatin-silk fibroin composite microspheres for mesenchymal stem cell expansion and osteogenic differentiation. Materials Today Communications, 44 (111912). |
| 19. Jianping Zhao, Di Zhang, Qiumei Lan, Gang Zhong, Yisi Liu, Nathaniel Holwell, Xiang Wang, Jinzhi Meng, Jun Yao, Brian G. Amsden, Yin Yu, Fei Chen. (2024) Tendon Decellularized Matrix Modified Fibrous Scaffolds with Porous and Crimped Microstructure for Tendon Regeneration. ACS Applied Bio Materials, 7 (7): (4747-4759). |
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