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CD8 alpha Rat mAb (AF750) - Primary antibody, specific to CD8 alpha

COA

Grade & Purity:

Validated

ExactAb™

0.5 mg/mL

Application:

Flow Cytometry

Host species: Rat
Reactive species: Mouse
Isotype: Rat IgG2a
Clone Number: 53-6.7
Conjugation: AF750

In stock

Item Number

Ab190222

Grouped product items
SKU	Size	Availability	Price
Ab190222-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$17.90
Ab190222-25μg	25μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$35.90
Ab190222-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$84.90

mAb (53-6.7); Rat anti Mouse CD8 alpha Antibody (AF750); Flow Cytometry

View related series

Click to view target information: CD8A

Accession#:P01731 (20) AF750 (22) Gene ID:12525 (20) Ly-2 (14) Ly-35 (13) Ly-B (13) Lyt-2 (16)

Basic Description

Product Name	CD8 alpha Rat mAb (AF750) - Primary antibody, specific to CD8 alpha
Synonyms	Ly-2 \| Ly-35 \| Ly-B \| Lyt-2 \| CD_antigen: CD8a \| CD8 antigen, alpha polypeptide (p32) \| CD8 \| CD8a molecule \| CD8A \| Leu2 T-lymphocyte antigen \| LEU2 \| MAL \| OKT8 T-cell antigen \| p32 \| T cell co-receptor \| T8 T-cell antigen \| T-cell antigen Leu2 \| T-cell
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Host species	Rat
Specificity	CD8 alpha
Immunogen	Mouse Spleen Cells or Thymocyte Membranes
Positive Control	Flow: C57BL/6 mouse splenocytes.
Conjugation	AF750
Excitation(nm)	Red Laser(633nm)
Emission(nm)	776nm
Ex/Em(nm)	Ex:752nm;Em:776nm
Grade	ExactAb™, Validated
Product Description	Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Product Properties

Light Chain Type	kappa
Purification Method	Protein G purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: CD8 alpha Rat mAb (AF750) (Ab190222)

CD8 alpha Rat mAb (AF750) (Ab190222) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (AF750) (Ab190222) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF750) (Ab190243).

Flow Cytometry: CD8 alpha Rat mAb (AF750) (Ab190222)

CD8 alpha Rat mAb (AF750) (Ab190222) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (AF750) (Ab190222) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF750) (Ab190243). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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